David Tscharke

2024

DOCK2 Deficiency Causes Defects in Antiviral T-Cell Responses and Impaired Control of Herpes Simplex Virus Infection

Randall, K. L., Flesch, I. E. A., Mei, Y., Miosge, L. A., Aye, R., Yu, Z., Domaschenz, H., Hollett, N. A., Russell, T. A., Stefanovic, T., Wong, Y. C., Seneviratne, S., Ballard, F., Hernandez Gallardo, R., Croft, S. N., Goodnow, C. C., Bertram, E. M., Enders, A. & Tscharke, D. C., 15 Feb 2024, In: Journal of Infectious Diseases. 10 p., jiae077.

Research output: Contribution to journal › Article › peer-review

Open Access

3 Citations (Scopus)

GPR41 and GPR43 regulate CD8⁺ T cell priming during herpes simplex virus type 1 infection

Lee, A. R., Wilson, K. R., Clarke, M., Engel, S., Tscharke, D. C., Gebhardt, T., Bedoui, S. & Bachem, A., 8 Mar 2024, In: Frontiers in Immunology. 15, 1332588.

Research output: Contribution to journal › Article › peer-review

Open Access

4 Citations (Scopus)

Lytic promoter activity during herpes simplex virus latency is dependent on genome location

Singh, N., Zachariah, S., T. Phillips, A. & Tscharke, D., 19 Nov 2024, In: Journal of Virology. 98, 11, 18 p.

Research output: Contribution to journal › Article › peer-review

Open Access

Multi-targeted loss of the antigen presentation molecule MR1 during HSV-1 and HSV-2 infection

Samer, C., McWilliam, H. E. G., McSharry, B. P., Velusamy, T., Burchfield, J. G., Stanton, R. J., Tscharke, D. C., Rossjohn, J., Villadangos, J. A., Abendroth, A. & Slobedman, B., 4 Jan 2024, In: iScience. 27, 2, 23 p., 108801.

Research output: Contribution to journal › Article › peer-review

Open Access

2 Citations (Scopus)

2023

BMX-A and BMX-S: Accessible cell-free methods to estimate peptide-MHC-I affinity and stability

Witney, M. J. & Tscharke, D. C., Sept 2023, In: Molecular Immunology. 161, p. 1-10 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

CTLA4 protects against maladaptive cytotoxicity during the differentiation of effector and follicular CD4⁺ T cells

Hao, Y., Miraghazadeh, B., Chand, R., Davies, A. R., Cardinez, C., Kwong, K., Downes, M. B., Sweet, R. A., Cañete, P. F., D’Orsogna, L. J., Fulcher, D. A., Choo, S., Yip, D., Peters, G., Yip, S., Witney, M. J., Nekrasov, M., Feng, Z. P., Tscharke, D. C., Vinuesa, C. G., & 1 othersCook, M. C., Jul 2023, In: Cellular and Molecular Immunology. 20, 7, p. 777-793 17 p.

Research output: Contribution to journal › Article › peer-review

Open Access

4 Citations (Scopus)

From bedside to bench: how existing therapies inform the relationship between Epstein–Barr virus and multiple sclerosis

Dyer, Z., Tscharke, D., Sutton, I. & Massey, J., 23 Feb 2023, In: Clinical and Translational Immunology. 12, 2, e1437.

Research output: Contribution to journal › Article › peer-review

Open Access

7 Citations (Scopus)

The expression and function of HSV ICP47 and its promoter in mice

Velusamy, T., Singh, N., Croft, S., Smith, S. & Tscharke, D. C., 30 Oct 2023, In: Journal of Virology. 97, 11 November, p. 1-21 21 p., 0110723.

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Varicella Zoster Virus Impairs Expression of the Nonclassical Major Histocompatibility Complex Class I-Related Gene Protein (MR1)

Purohit, S. K., Samer, C., McWilliam, H. E. G., Traves, R., Steain, M., McSharry, B. P., Kinchington, P. R., Tscharke, D. C., Villadangos, J. A., Rossjohn, J., Abendroth, A. & Slobedman, B., 1 Feb 2023, In: Journal of Infectious Diseases. 227, 3, p. 391-401 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

12 Citations (Scopus)

2022

FROM CALIGARI TO JOKER: the clown prince of crime’s psychopathic science

Jürgens, D. A. S., Tscharke, P. D. & Brocks, P. J., 2022, In: Journal of Graphic Novels and Comics. 13, 5, p. 685-699 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

8 Citations (Scopus)

Shielding Surfaces from Viruses and Bacteria with a Multiscale Coating

Ashok, D., Taheri, M., Garg, P., Webb, D., Parajuli, P., Wang, Y., Funnell, B., Taylor, B., Tscharke, D. C., Tsuzuki, T., Verma, N. K., Tricoli, A. & Nisbet, D. R., 15 Aug 2022, In: Advanced Science. 9, 23, 2201415.

Research output: Contribution to journal › Article › peer-review

Open Access

11 Citations (Scopus)

2021

Direct priming of CD8+ T cells persists in the face of cowpox virus inhibitors of antigen presentation

Lin, L. C. W., Croft, S. N., Croft, N. P., Wong, Y. C., Smith, S. A., Tang, S. S., Purcell, A. W. & Tscharke, D. C., May 2021, In: Journal of Virology. 95, 10, e00186-21.

Research output: Contribution to journal › Article › peer-review

Open Access

2 Citations (Scopus)

Ptpn2 and KLRG1 regulate the generation and function of tissue-resident memory CD8⁺ T cells in skin

Hochheiser, K., Wiede, F., Wagner, T., Freestone, D., Enders, M. H., Olshansky, M., Russ, B., Nüssing, S., Bawden, E., Braun, A., Bachem, A., Gressier, E., McConville, R., Park, S. L., Jones, C. M., Davey, G. M., Gyorki, D. E., Tscharke, D., Parish, I. A., Turner, S., & 4 othersHerold, M. J., Tiganis, T., Bedoui, S. & Gebhardt, T., 29 Apr 2021, In: Journal of Experimental Medicine. 218, 6, e20200940.

Research output: Contribution to journal › Article › peer-review

Open Access

16 Citations (Scopus)

Selection of Vaccinia Virus Recombinants Using CRISPR/Cas9

Gowripalan, A., Smith, S. A. & Tscharke, D. C., 20 Dec 2021, In: Bio-protocol. 11, 24, e4270.

Research output: Contribution to journal › Article › peer-review

Open Access

6 Citations (Scopus)

Spreading Fun: Comic Zombies, Joker Viruses and COVID-19 Jokes

Jurgens, A-S., Fiadotava, A., Tscharke, D. & Viana, J. N., 2021, In: Journal of Science and Popular Culture. 4, 1, p. 39-57

Research output: Contribution to journal › Article › peer-review

Viperin binds STING and enhances the type-I interferon response following dsDNA detection

Crosse, K. M., Monson, E. A., Dumbrepatil, A. B., Smith, M., Tseng, Y. Y., Van der Hoek, K. H., Revill, P. A., Saker, S., Tscharke, D. C., G Marsh, E. N., Beard, M. R. & Helbig, K. J., Apr 2021, In: Immunology and Cell Biology. 99, 4, p. 373-391 19 p.

Research output: Contribution to journal › Article › peer-review

Open Access

27 Citations (Scopus)

Viperin has species-specific roles in response to herpes simplex virus infection

Tseng, Y. Y., Gowripalan, A., Croft, S. N., Smith, S. A., Helbig, K. J., Man, S. M. & Tscharke, D. C., 2021, In: Journal of General Virology. 102, 8, 001638.

Research output: Contribution to journal › Article › peer-review

Open Access

4 Citations (Scopus)

2020

Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome

Fox, D., Mathur, A., Xue, Y., Liu, Y., Tan, W. H., Feng, S., Pandey, A., Ngo, C., Hayward, J. A., Atmosukarto, I. I., Price, J. D., Johnson, M. D., Jessberger, N., Robertson, A. A. B., Burgio, G., Tscharke, D. C., Fox, E. M., Leyton, D. L., Kaakoush, N. O., Märtlbauer, E., & 2 othersLeppla, S. H. & Man, S. M., 1 Dec 2020, In: Nature Communications. 11, 1, 760.

Research output: Contribution to journal › Article › peer-review

Open Access

62 Citations (Scopus)

Benchmarking predictions of MHC class I restricted T cell epitopes in a comprehensively studied model system

Paul, S., Croft, N. P., Purcell, A. W., Tscharke, D. C., Sette, A., Nielsen, M. & Peters, B., May 2020, In: PLoS Computational Biology. 16, 5, e1007757.

Research output: Contribution to journal › Article › peer-review

Open Access

57 Citations (Scopus)

Herpes simplex virus latency is noisier the closer we look

Singh, N. & Tscharke, D. C., 1 Feb 2020, In: Journal of Virology. 94, 4, e01701-19.

Research output: Contribution to journal › Article › peer-review

Open Access

64 Citations (Scopus)

Overlapping peptides elicit distinct CD8⁺T cell responses following influenza a virus infection

Assmus, L. M., Guan, J., Wu, T., Farenc, C., Sng, X. Y. X., Zareie, P., Nguyen, A., Nguyen, A. T., Tscharke, D. C., Thomas, P. G., Rossjohn, J., Gras, S., Croft, N. P., Purcell, A. W. & Gruta, N. L. L., 1 Oct 2020, In: Journal of Immunology. 205, 7, p. 1731-1742 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

9 Citations (Scopus)

Rapid poxvirus engineering using CRISPR/Cas9 as a selection tool

Gowripalan, A., Smith, S., Stefanovic, T. & Tscharke, D. C., 1 Dec 2020, In: Communications Biology. 3, 1, 643.

Research output: Contribution to journal › Article › peer-review

Open Access

21 Citations (Scopus)

Surprisingly Effective Priming of CD8⁺ T Cells by Heat-Inactivated Vaccinia Virus Virions

Croft, S., Wong, Y. C., Smith, S. A., Flesch, I. E. A. & Tscharke, D. C., 29 Sept 2020, In: Journal of Virology. 94, 20, e01486-20.

Research output: Contribution to journal › Article › peer-review

Open Access

8 Citations (Scopus)

2019

Functional paralysis of human natural killer cells by alphaherpesviruses

Campbell, T. M., McSharry, B. P., Steain, M., Russell, T. A., Tscharke, D. C., Kennedy, J. J., Slobedman, B. & Abendroth, A., Jun 2019, In: PLoS Pathogens. 15, 6, e1007784.

Research output: Contribution to journal › Article › peer-review

Open Access

20 Citations (Scopus)

Influenza A virus infection induces viral and cellular defective ribosomal products encoded by alternative reading frames

Zanker, D. J., Oveissi, S., Tscharke, D. C., Duan, M., Wan, S., Zhang, X., Xiao, K., Mifsud, N. A., Gibbs, J., Izzard, L., Dlugolenski, D., Faou, P., Laurie, K. L., Vigneron, N., Barr, I. G., Stambas, J., Van Den Eynde, B. J., Bennink, J. R., Yewdell, J. W. & Chen, W., 15 Jun 2019, In: Journal of Immunology. 202, 12, p. 3370-3380 11 p.

Research output: Contribution to journal › Article › peer-review

Open Access

24 Citations (Scopus)

Modified vaccinia virus Ankara can induce optimal CD8⁺ T cell responses to directly primed antigens depending on vaccine design

Wong, Y. C., Croft, S., Smith, S. A., Lin, L. C. W., Cukalac, T., la Gruta, N. L., Drexler, I. & Tscharke, D. C., 2019, In: Journal of Virology. 93, 21, e01154-19.

Research output: Contribution to journal › Article › peer-review

Open Access

15 Citations (Scopus)

Most viral peptides displayed by class I MHC on infected cells are immunogenic

Croft, N. P., Smith, S. A., Pickering, J., Sidney, J., Peters, B., Faridi, P., Witney, M. J., Sebastian, P., Flesch, I. E. A., Heading, S. L., Sette, A., La Gruta, N. L., Purcell, A. W. & Tscharke, D. C., 19 Feb 2019, In: Proceedings of the National Academy of Sciences of the United States of America. 116, 8, p. 3112-3117 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

92 Citations (Scopus)

Quantification of epitope abundance reveals the effect of direct and cross-presentation on influenza CTL responses

Wu, T., Guan, J., Handel, A., Tscharke, D. C., Sidney, J., Sette, A., Wakim, L. M., Sng, X. Y. X., Thomas, P. G., Croft, N. P., Purcell, A. W. & La Gruta, N. L., 1 Dec 2019, In: Nature Communications. 10, 1, 2846.

Research output: Contribution to journal › Article › peer-review

Open Access

76 Citations (Scopus)

2018

Effective priming of herpes simplex virusspecific CD8⁺ T cells in vivo does not require infected dendritic cells

Whitney, P. G., Makhlouf, C., MacLeod, B., Ma, J. Z., Gressier, E., Greyer, M., Hochheiser, K., Bachem, A., Zaid, A., Voehringer, D., Heath, W. R., Wagle, M. V., Parish, I., Russell, T. A., Smith, S. A., Tscharke, D. C., Gebhardt, T. & Bedoui, S., 1 Feb 2018, In: Journal of Virology. 92, 3, e01508-17.

Research output: Contribution to journal › Article › peer-review

Open Access

14 Citations (Scopus)

Increasing antigen presentation on HSV-1-infected cells increases lesion size but does not alter neural infection or latency

Russell, T. A., Velusamy, T., Tseng, Y. Y. & Tscharke, D. C., May 2018, In: Journal of General Virology. 99, 5, p. 682-692 11 p., 001059.

Research output: Contribution to journal › Article › peer-review

Open Access

2 Citations (Scopus)

Local proliferation maintains a stable pool of tissue-resident memory T cells after antiviral recall responses article

Park, S. L., Zaid, A., Hor, J. L., Christo, S. N., Prier, J. E., Davies, B., Alexandre, Y. O., Gregory, J. L., Russell, T. A., Gebhardt, T., Carbone, F. R., Tscharke, D. C., Heath, W. R., Mueller, S. N. & MacKay, L. K., 1 Feb 2018, In: Nature Immunology. 19, 2, p. 183-191 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

252 Citations (Scopus)

2017

An ultrastructural investigation of tumors undergoing regression mediated by immunotherapy

Westwood, J. A., Ellis, S., Danne, J., Johnson, C., Oorschot, V., Ramm, G., Tscharke, D. C., Davenport, A. J., Whisstock, J. C., Darcy, P. K., Kershaw, M. H. & Slaney, C. Y., 2017, In: Oncotarget. 8, 70, p. 115215-115229 15 p.

Research output: Contribution to journal › Article › peer-review

Open Access

6 Citations (Scopus)

Dual-specific chimeric antigen receptor T cells and an indirect vaccine eradicate a variety of large solid tumors in an immunocompetent, self-antigen setting

Slaney, C. Y., Von Scheidt, B., Davenport, A. J., Beavis, P. A., Westwood, J. A., Mardiana, S., Tscharke, D. C., Ellis, S., Prince, H. M., Trapani, J. A., Johnstone, R. W., Smyth, M. J., Teng, M. W., Ali, A., Yu, Z., Rosenberg, S. A., Restifo, N. P., Neeson, P., Darcy, P. K. & Kershaw, M. H., 15 May 2017, In: Clinical Cancer Research. 23, 10, p. 2478-2490 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

91 Citations (Scopus)

Ectromelia virus N1L is essential for virulence but not dissemination in a classical model of mousepox

Melo-Silva, C. R., Tscharke, D. C., Lobigs, M., Koskinen, A., Müllbacher, A. & Regner, M., 15 Jan 2017, In: Virus Research. 228, p. 61-65 5 p.

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Systems-guided forward genetic screen reveals a critical role of the replication stress response protein ETAA1 in T cell clonal expansion

Miosge, L. A., Sontani, Y., Chuah, A., Horikawa, K., Russell, T. A., Meia, Y., Wagle, M. V., Howard, D. R., Enders, A., Tscharke, D. C., Goodnow, C. C. & Parish, I. A., 27 Jun 2017, In: Proceedings of the National Academy of Sciences of the United States of America. 114, 26, p. E5216-E5225

Research output: Contribution to journal › Article › peer-review

Open Access

15 Citations (Scopus)

2016

Lytic Promoters Express Protein during Herpes Simplex Virus Latency

Russell, T. A. & Tscharke, D. C., Jun 2016, In: PLoS Pathogens. 12, 6, e1005729.

Research output: Contribution to journal › Article › peer-review

Open Access

31 Citations (Scopus)

The Epstein-Barr Virus Glycoprotein gp150 Forms an Immune-Evasive Glycan Shield at the Surface of Infected Cells

Gram, A. M., Oosenbrug, T., Lindenbergh, M. F. S., Büll, C., Comvalius, A., Dickson, K. J. I., Wiegant, J., Vrolijk, H., Lebbink, R. J., Wolterbeek, R., Adema, G. J., Griffioen, M., Heemskerk, M. H. M., Tscharke, D. C., Hutt-Fletcher, L. M., Wiertz, E. J. H. J., Hoeben, R. C. & Ressing, M. E., Apr 2016, In: PLoS Pathogens. 12, 4, e1005550.

Research output: Contribution to journal › Article › peer-review

Open Access

24 Citations (Scopus)

The primary immune response to Vaccinia virus vaccination includes cells with a distinct cytotoxic effector CD4 T-cell phenotype

Munier, C. M. L., van Bockel, D., Bailey, M., Ip, S., Xu, Y., Alcantara, S., Liu, S. M., Denyer, G., Kaplan, W., Suzuki, K., Croft, N., Purcell, A., Tscharke, D., Cooper, D. A., Kent, S. J., Zaunders, J. J. & Kelleher, A. D., 17 Oct 2016, In: Vaccine. 34, 44, p. 5251-5261 11 p.

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

2015

Delayed control of herpes simplex virus infection and impaired CD4⁺ T-cell migration to the skin in mouse models of DOCK8 deficiency

Flesch, I. E. A., Randall, K. L., Hollett, N. A., Di Law, H., Miosge, L. A., Sontani, Y., Goodnow, C. C. & Tscharke, D. C., 8 Jul 2015, In: Immunology and Cell Biology. 93, 6, p. 517-521 5 p.

Research output: Contribution to journal › Article › peer-review

Open Access

15 Citations (Scopus)

Engineering herpes simplex viruses by infection-transfection methods including recombination site targeting by CRISPR/Cas9 nucleases

Russell, T. A., Stefanovic, T. & Tscharke, D. C., 1 Mar 2015, In: Journal of Virological Methods. 213, p. 18-25 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

73 Citations (Scopus)

Extent of systemic spread determines CD8⁺ T cell immunodominance for laboratory strains, smallpox vaccines, and zoonotic isolates of vaccinia virus

Flesch, I. E. A., Hollett, N. A., Wong, Y. C., Quinan, B. R., Howard, D., Da Fonseca, F. G. & Tscharke, D. C., 1 Sept 2015, In: Journal of Immunology. 195, 5, p. 2263-2272 10 p.

Research output: Contribution to journal › Article › peer-review

Open Access

11 Citations (Scopus)

Functional analysis of the short isoform of orf virus protein OV20.0

Tseng, Y. Y., Lin, F. Y., Cheng, S. F., Tscharke, D., Chulakasian, S., Chou, C. C., Liu, Y. F., Chang, W. S., Wong, M. L. & Hsu, W. L., 2015, In: Journal of Virology. 89, 9, p. 4966-4979 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

15 Citations (Scopus)

Redundancy complicates the definition of essential genes for vaccinia virus

Dobson, B. M. & Tscharke, D. C., Nov 2015, In: Journal of General Virology. 96, 11, p. 3326-3337 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

12 Citations (Scopus)

Simultaneous quantification of viral antigen expression kinetics using data-independent (DIA) mass spectrometry

Croft, N. P., De Verteuil, D. A., Smith, S. A., Wong, Y. C., Schittenhelm, R. B., Tscharke, D. C. & Purcell, A. W., 1 May 2015, In: Molecular and Cellular Proteomics. 14, 5, p. 1361-1372 12 p.

Research output: Contribution to journal › Article › peer-review

Open Access

22 Citations (Scopus)

2014

An intact signal peptide on dengue virus E protein enhances immunogenicity for CD8⁺ T cells and antibody when expressed from modified vaccinia Ankara

Quinan, B. R., Flesch, I. E. A., Pinho, T. M. G., Coelho, F. M., Tscharke, D. C. & da Fonseca, F. G., 23 May 2014, In: Vaccine. 32, 25, p. 2972-2979 8 p.

Research output: Contribution to journal › Article › peer-review

Open Access

17 Citations (Scopus)

Distinct APC Subtypes Drive Spatially Segregated CD4⁺ and CD8⁺ T-Cell Effector Activity during Skin Infection with HSV-1

Macleod, B. L., Bedoui, S., Hor, J. L., Mueller, S. N., Russell, T. A., Hollett, N. A., Heath, W. R., Tscharke, D. C., Brooks, A. G. & Gebhardt, T., 14 Aug 2014, In: PLoS Pathogens. 10, 8, e1004303.

Research output: Contribution to journal › Article › peer-review

Open Access

43 Citations (Scopus)

Lytic Gene Expression Is Frequent in HSV-1 Latent Infection and Correlates with the Engagement of a Cell-Intrinsic Transcriptional Response

Ma, J. Z., Russell, T. A., Spelman, T., Carbone, F. R. & Tscharke, D. C., Jul 2014, In: PLoS Pathogens. 10, 7, e1004237.

Research output: Contribution to journal › Article › peer-review

Open Access

67 Citations (Scopus)

Strikingly poor CD8 + T-cell immunogenicity of vaccinia virus strain MVA in BALB/c mice

Russell, T. A. & Tscharke, D. C., 2014, In: Immunology and Cell Biology. 92, 5, p. 466-469 4 p.

Research output: Contribution to journal › Article › peer-review

Open Access

13 Citations (Scopus)

The cellular redox environment alters antigen presentation

Trujillo, J. A., Croft, N. P., Dudek, N. L., Channappanavar, R., Theodossis, A., Webb, A. I., Dunstone, M. A., Illing, P. T., Butler, N. S., Fett, C., Tscharke, D. C., Rossjohn, J., Perlman, S. & Purcell, A. W., 3 Oct 2014, In: Journal of Biological Chemistry. 289, 40, p. 27979-27991 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

47 Citations (Scopus)

Truncation of gene F5L partially masks rescue of vaccinia virus strain MVA growth on mammalian cells by restricting plaque size

Dobson, B. M. & Tscharke, D. C., Feb 2014, In: Journal of General Virology. 95, PART 2, p. 466-471 6 p.

Research output: Contribution to journal › Article › peer-review

Open Access

7 Citations (Scopus)

Prof David Tscharke

Research output

Search results