3D structure determination of biomolecular assemblies from sparse data of different length scales

    Project: Research

    Project Details

    Description

    The 3D structure of proteins is essential for their function but is also often difficult to determine. This project will develop new computer algorithms to generate accurate 3D models of large protein-protein and protein-ligand complexes from sparse experimental structure restraints. Advanced protein chemistry techniques will be used to introduce unnatural amino acids and tag them with novel lanthanide or cross-linking reagents. Structure restraints will be collected by solution and solid-state NMR, EPR and mass spectrometry. New strategies and algorithms will open the way to using this approach with RNA and exceptionally large protein assemblies. The techniques will be developed and tested on proteins that are established drug targets.
    StatusFinished
    Effective start/end date1/01/1231/12/15

    Funding

    • Australian Research Council (ARC): A$610,000.00

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