A fast fluorescence lifetime imaging microscope to track protein dynamics

Here we aim to establish an optical microscope that can perform single particle tracking experiments throughoutthe entire structural framework of a living cell and fast fluorescence lifetime imaging microscopy of Frsterresonance energy transfer. Collectively this research equipment will have sufficient spatiotemporal resolution tointerrogate how a protein navigates intracellular architecture and when versus where a protein interacts with subcellularsubstrates. Establishing this technology in Australia will enable researchers to interrogate at the singlemolecule level, how a proteins journey throughout the cellular landscape regulates complex biological functionsessential to cellular homeostasis.