Identifying potential barriers to transplanting modified forms of the CO2-fixing enzyme, Rubisco, into plants

    Project: Research

    Project Details

    Description

    Rubisco is the primary CO2-fixing enzyme, but its slow catalysis often limits photosynthesis. The engineering of more efficient Rubiscos into plants, to improve growth, requires complete understanding of its synthesis and regulation in chloroplasts. Specific amino acid residues within the Rubisco large subunit N terminal coding sequence are proposed to influence its translation, stability and interaction capacity with its regulatory protein, Rubisco activase. Using our novel tobacco chloroplast transformation system, the influence of particular sections of N-terminus sequence in these processes will be examined. This will ascertain the permissible extent of modification, information which is critical to Rubisco engineering strategies.
    StatusFinished
    Effective start/end date1/01/091/06/12

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