New Methods for Structural Biology in Solution

    Project: Research

    Project Details

    Description

    3D structure underpins protein function but remains difficult to elucidate in many cases. This application is for the development of new nuclear magnetic resonance (NMR) and cell-free protein production technologies to characterize the structure of the functionally important parts of proteins. The project encompasses development of a new method for protein structure determination using lanthanide-labelling, chemical synthesis of lanthanide-binding tags, high-throughput cell-free protein production, and applications to 3D structure determinations of large (>100 kDa) protein assemblies and of protein-ligand complexes. Targets include the dengue virus NS2B/NS3 protease. The project aims for immediate impact in pharmaceutical industries.
    StatusFinished
    Effective start/end date1/01/0831/12/10

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