New methods for structure analysis of proteins and protein interactions

    Project: Research

    Project Details

    Description

    3D structure underpins protein function but remains difficult to elucidate in many cases. This project will advance nuclear magnetic resonance (NMR) technologies pioneered at the ANU which are based on paramagnetic metal tags. A new and diverse set of strategies for site-specific tagging of proteins with lanthanides or Co(II) will dramatically extend the range of applications, including fast 3D structure determinations of proteins by NMR and structure analysis of large complexes by electron paramagnetic resonance (EPR). Combined with 13C-NMR of perdeuterated proteins and a novel, uniquely flexible segmental isotope labelling strategy, the tags will be applied to targets of interest in the fight against cancer and bacterial infections.
    StatusFinished
    Effective start/end date1/01/1131/12/14

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