Nuclear RNA surveillance and its connection to splicing quality control

Due to the error-prone nature of RNA splicing, elaborate quality control processes ensure that only correctlyspliced transcripts can leave the nucleus. It has long been known that incorrectly spliced mRNA transcripts aredegraded by the nuclear RNA surveillance machinery, but how the RNA quality control machinery is connected tonuclear RNA surveillance is not known. This proposal aims to uncover the connection between these twoimportant processes and will fill a significant gap in our understanding of how splicing quality control and nuclearRNA surveillance work. The project will also identify sequence features that trigger abortive splicing reactions andwill thus help to improve the design of synthetic mRNAs.