13c isotope labelling to follow the flux of photorespiratory intermediates

Cyril Abadie, Guillaume Tcherkez*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Measuring the carbon flux through metabolic pathways in intact illuminated leaves re-mains challenging because of, e.g., isotopic dilution by endogenous metabolites, the impossibility to reach isotopic steady state, and the occurrence of multiple pools. In the case of photorespiratory intermediates, our knowledge of the partitioning between photorespiratory recycling, storage, and utilization by other pathways is thus rather limited. There has been some controversy as to whether photorespiratory glycine and serine may not be recycled, thus changing the apparent stoichiometric coefficient between photorespiratory O2 fixation and CO2 release. We describe here an isotopic method to trace the fates of glycine, serine and glycerate, taking advantage of positional13C content with NMR and isotopic analyses by LC–MS. This technique is well-adapted to show that the pro-portion of glycerate, serine and glycine molecules escaping photorespiratory recycling is very small.

    Original languageEnglish
    Article number427
    Pages (from-to)1-12
    Number of pages12
    JournalPlants
    Volume10
    Issue number3
    DOIs
    Publication statusPublished - Mar 2021

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