3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

Bin Bin Pan; Feng Yang; Yansheng Ye; Qiong Wu; Conggang Li; Thomas Huber; Xun Cheng Su

doi:10.1039/c6cc05490k

3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

Bin Bin Pan
, Feng Yang
, Yansheng Ye
, Qiong Wu
, Conggang Li
, Thomas Huber
, Xun Cheng Su^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

96 Citations (Scopus)

Abstract

Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes.

Original language	English
Pages (from-to)	10237-10240
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	67
DOIs	https://doi.org/10.1039/c6cc05490k
Publication status	Published - 2016

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title = "3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy",

abstract = "Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes.",

author = "Pan, \{Bin Bin\} and Feng Yang and Yansheng Ye and Qiong Wu and Conggang Li and Thomas Huber and Su, \{Xun Cheng\}",

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year = "2016",

doi = "10.1039/c6cc05490k",

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T1 - 3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

AU - Pan, Bin Bin

AU - Yang, Feng

AU - Ye, Yansheng

AU - Wu, Qiong

AU - Li, Conggang

AU - Huber, Thomas

AU - Su, Xun Cheng

PY - 2016

Y1 - 2016

N2 - Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes.

AB - Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein-ligand complexes.

UR - https://www.scopus.com/pages/publications/84982170102

U2 - 10.1039/c6cc05490k

DO - 10.1039/c6cc05490k

M3 - Article

SN - 1359-7345

VL - 52

SP - 10237

EP - 10240

JO - Chemical Communications

JF - Chemical Communications

IS - 67

ER -

3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

Abstract

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