4-picoline-2,2′:6′,2″-terpyridine-platinum(II) - A potent intercalator of DNA

Angela McCoubrey; Harriet C. Latham; Peter R. Cook; Alison Rodger; Gordon Lowe

doi:10.1016/0014-5793(95)01537-X

4-picoline-2,2′:6′,2″-terpyridine-platinum(II) - A potent intercalator of DNA

Angela McCoubrey, Harriet C. Latham, Peter R. Cook, Alison Rodger, Gordon Lowe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

4-Picoline-2,2′:6′,2″-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 × 10⁷ M^-1 for the most stable binding mode of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) to poly[d(A-T)₂] with a site size of about 4 base pairs, and about 1 × 10⁶ M^-1 for a second binding mode with a site size of about 2 base pairs. Fluorescence spectroscopy provides further evidence for the strong equilibrium binding constant of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) in that it displaces ethidium bromide bound to DNA. The double positive charge on 4-picoline-2,2′:6′,2″-terpyridine-platinum(II), together with the intercalative binding mode is probably responsible for the large binding constant.

Original language	English
Pages (from-to)	73-78
Number of pages	6
Journal	FEBS Letters
Volume	380
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(95)01537-X
Publication status	Published - 12 Feb 1996
Externally published	Yes

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title = "4-picoline-2,2′:6′,2″-terpyridine-platinum(II) - A potent intercalator of DNA",

abstract = "4-Picoline-2,2′:6′,2″-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 × 107 M-1 for the most stable binding mode of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) to poly[d(A-T)2] with a site size of about 4 base pairs, and about 1 × 106 M-1 for a second binding mode with a site size of about 2 base pairs. Fluorescence spectroscopy provides further evidence for the strong equilibrium binding constant of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) in that it displaces ethidium bromide bound to DNA. The double positive charge on 4-picoline-2,2′:6′,2″-terpyridine-platinum(II), together with the intercalative binding mode is probably responsible for the large binding constant.",

keywords = "Circular dichroism, DNA ligation, Fluorescence spectroscopy",

author = "Angela McCoubrey and Latham, {Harriet C.} and Cook, {Peter R.} and Alison Rodger and Gordon Lowe",

year = "1996",

month = feb,

day = "12",

doi = "10.1016/0014-5793(95)01537-X",

language = "English",

volume = "380",

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T1 - 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) - A potent intercalator of DNA

AU - McCoubrey, Angela

AU - Latham, Harriet C.

AU - Cook, Peter R.

AU - Rodger, Alison

AU - Lowe, Gordon

PY - 1996/2/12

Y1 - 1996/2/12

N2 - 4-Picoline-2,2′:6′,2″-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 × 107 M-1 for the most stable binding mode of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) to poly[d(A-T)2] with a site size of about 4 base pairs, and about 1 × 106 M-1 for a second binding mode with a site size of about 2 base pairs. Fluorescence spectroscopy provides further evidence for the strong equilibrium binding constant of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) in that it displaces ethidium bromide bound to DNA. The double positive charge on 4-picoline-2,2′:6′,2″-terpyridine-platinum(II), together with the intercalative binding mode is probably responsible for the large binding constant.

AB - 4-Picoline-2,2′:6′,2″-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 × 107 M-1 for the most stable binding mode of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) to poly[d(A-T)2] with a site size of about 4 base pairs, and about 1 × 106 M-1 for a second binding mode with a site size of about 2 base pairs. Fluorescence spectroscopy provides further evidence for the strong equilibrium binding constant of 4-picoline-2,2′:6′,2″-terpyridine-platinum(II) in that it displaces ethidium bromide bound to DNA. The double positive charge on 4-picoline-2,2′:6′,2″-terpyridine-platinum(II), together with the intercalative binding mode is probably responsible for the large binding constant.

KW - Circular dichroism

KW - DNA ligation

KW - Fluorescence spectroscopy

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DO - 10.1016/0014-5793(95)01537-X

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AN - SCOPUS:0030067055

SN - 0014-5793

VL - 380

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JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

4-picoline-2,2′:6′,2″-terpyridine-platinum(II) - A potent intercalator of DNA

Abstract

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