A bedside assay to detect streptococcus pneumoniae in children with empyema

Roxanne E. Strachan; Anita Cornelius; Gwendolyn L. Gilbert; Tanya Gulliver; Andrew Martin; Tim McDonald; Gillian M. Nixon; Rob Roseby; Sarath Ranganathan; Hiran Selvadurai; Greg Smith; Manuel Soto-Martinez; Sadasivam Suresh; Laurel Teoh; Kiran Thapa; Claire E. Wainwright; Adam Jaffé

doi:10.1002/ppul.21349

A bedside assay to detect streptococcus pneumoniae in children with empyema

Roxanne E. Strachan, Anita Cornelius, Gwendolyn L. Gilbert, Tanya Gulliver, Andrew Martin, Tim McDonald, Gillian M. Nixon, Rob Roseby, Sarath Ranganathan, Hiran Selvadurai, Greg Smith, Manuel Soto-Martinez, Sadasivam Suresh, Laurel Teoh, Kiran Thapa, Claire E. Wainwright, Adam Jaffé^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Background: Empyema is a complication of pneumonia, commonly caused by Streptococcus pneumoniae. Aims To validate the utility of an immunochromatographic test for the detection of S. pneumoniae antigen in the pleural fluid of children with empyema. Methods Empyema patients had blood and pleural fluid cultured, and polymerase chain reaction (PCR) to detect the S. pneumoniae autolysin gene, lytA, in pleural fluid. Pleural fluid was tested using the Binax NOW S. pneumoniae antigen detection assay and compared with lytA PCR results and/or culture in blood or pleural fluid. Results S. pneumoniae was detected by PCR in pleural fluid of 68 of 137 (49.6%) patients, by culture in 11 of 135 (8.1%) pleural specimens and 16 of 120 (13.3%) blood specimens. Pleural fluid Binax NOW testing from 130 patients demonstrated a sensitivity of 83.8% and specificity of 93.5% (positive predictive value of 93.4% and negative predictive value of 84.1%). Conclusions: In pediatric empyema, high predictive values of pleural fluid Binax NOW S. pneumoniae antigen test suggest that this test may help rationalize antibiotic choice in these patients.

Original language	English
Pages (from-to)	179-183
Number of pages	5
Journal	Pediatric Pulmonology
Volume	46
Issue number	2
DOIs	https://doi.org/10.1002/ppul.21349
Publication status	Published - Feb 2011
Externally published	Yes

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Strachan, R. E., Cornelius, A., Gilbert, G. L., Gulliver, T., Martin, A., McDonald, T., Nixon, G. M., Roseby, R., Ranganathan, S., Selvadurai, H., Smith, G., Soto-Martinez, M., Suresh, S., Teoh, L., Thapa, K., Wainwright, C. E., & Jaffé, A. (2011). A bedside assay to detect streptococcus pneumoniae in children with empyema. Pediatric Pulmonology, 46(2), 179-183. https://doi.org/10.1002/ppul.21349

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title = "A bedside assay to detect streptococcus pneumoniae in children with empyema",

abstract = "Background: Empyema is a complication of pneumonia, commonly caused by Streptococcus pneumoniae. Aims To validate the utility of an immunochromatographic test for the detection of S. pneumoniae antigen in the pleural fluid of children with empyema. Methods Empyema patients had blood and pleural fluid cultured, and polymerase chain reaction (PCR) to detect the S. pneumoniae autolysin gene, lytA, in pleural fluid. Pleural fluid was tested using the Binax NOW S. pneumoniae antigen detection assay and compared with lytA PCR results and/or culture in blood or pleural fluid. Results S. pneumoniae was detected by PCR in pleural fluid of 68 of 137 (49.6%) patients, by culture in 11 of 135 (8.1%) pleural specimens and 16 of 120 (13.3%) blood specimens. Pleural fluid Binax NOW testing from 130 patients demonstrated a sensitivity of 83.8% and specificity of 93.5% (positive predictive value of 93.4% and negative predictive value of 84.1%). Conclusions: In pediatric empyema, high predictive values of pleural fluid Binax NOW S. pneumoniae antigen test suggest that this test may help rationalize antibiotic choice in these patients.",

keywords = "Streptococcus pneumoniae, pediatric, pleural empyema, polymerase chain reaction",

author = "Strachan, {Roxanne E.} and Anita Cornelius and Gilbert, {Gwendolyn L.} and Tanya Gulliver and Andrew Martin and Tim McDonald and Nixon, {Gillian M.} and Rob Roseby and Sarath Ranganathan and Hiran Selvadurai and Greg Smith and Manuel Soto-Martinez and Sadasivam Suresh and Laurel Teoh and Kiran Thapa and Wainwright, {Claire E.} and Adam Jaff{\'e}",

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doi = "10.1002/ppul.21349",

language = "English",

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Strachan, RE, Cornelius, A, Gilbert, GL, Gulliver, T, Martin, A, McDonald, T, Nixon, GM, Roseby, R, Ranganathan, S, Selvadurai, H, Smith, G, Soto-Martinez, M, Suresh, S, Teoh, L, Thapa, K, Wainwright, CE & Jaffé, A 2011, 'A bedside assay to detect streptococcus pneumoniae in children with empyema', Pediatric Pulmonology, vol. 46, no. 2, pp. 179-183. https://doi.org/10.1002/ppul.21349

TY - JOUR

T1 - A bedside assay to detect streptococcus pneumoniae in children with empyema

AU - Strachan, Roxanne E.

AU - Cornelius, Anita

AU - Gilbert, Gwendolyn L.

AU - Gulliver, Tanya

AU - Martin, Andrew

AU - McDonald, Tim

AU - Nixon, Gillian M.

AU - Roseby, Rob

AU - Ranganathan, Sarath

AU - Selvadurai, Hiran

AU - Smith, Greg

AU - Soto-Martinez, Manuel

AU - Suresh, Sadasivam

AU - Teoh, Laurel

AU - Thapa, Kiran

AU - Wainwright, Claire E.

AU - Jaffé, Adam

PY - 2011/2

Y1 - 2011/2

N2 - Background: Empyema is a complication of pneumonia, commonly caused by Streptococcus pneumoniae. Aims To validate the utility of an immunochromatographic test for the detection of S. pneumoniae antigen in the pleural fluid of children with empyema. Methods Empyema patients had blood and pleural fluid cultured, and polymerase chain reaction (PCR) to detect the S. pneumoniae autolysin gene, lytA, in pleural fluid. Pleural fluid was tested using the Binax NOW S. pneumoniae antigen detection assay and compared with lytA PCR results and/or culture in blood or pleural fluid. Results S. pneumoniae was detected by PCR in pleural fluid of 68 of 137 (49.6%) patients, by culture in 11 of 135 (8.1%) pleural specimens and 16 of 120 (13.3%) blood specimens. Pleural fluid Binax NOW testing from 130 patients demonstrated a sensitivity of 83.8% and specificity of 93.5% (positive predictive value of 93.4% and negative predictive value of 84.1%). Conclusions: In pediatric empyema, high predictive values of pleural fluid Binax NOW S. pneumoniae antigen test suggest that this test may help rationalize antibiotic choice in these patients.

AB - Background: Empyema is a complication of pneumonia, commonly caused by Streptococcus pneumoniae. Aims To validate the utility of an immunochromatographic test for the detection of S. pneumoniae antigen in the pleural fluid of children with empyema. Methods Empyema patients had blood and pleural fluid cultured, and polymerase chain reaction (PCR) to detect the S. pneumoniae autolysin gene, lytA, in pleural fluid. Pleural fluid was tested using the Binax NOW S. pneumoniae antigen detection assay and compared with lytA PCR results and/or culture in blood or pleural fluid. Results S. pneumoniae was detected by PCR in pleural fluid of 68 of 137 (49.6%) patients, by culture in 11 of 135 (8.1%) pleural specimens and 16 of 120 (13.3%) blood specimens. Pleural fluid Binax NOW testing from 130 patients demonstrated a sensitivity of 83.8% and specificity of 93.5% (positive predictive value of 93.4% and negative predictive value of 84.1%). Conclusions: In pediatric empyema, high predictive values of pleural fluid Binax NOW S. pneumoniae antigen test suggest that this test may help rationalize antibiotic choice in these patients.

KW - Streptococcus pneumoniae

KW - pediatric

KW - pleural empyema

KW - polymerase chain reaction

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U2 - 10.1002/ppul.21349

DO - 10.1002/ppul.21349

M3 - Article

SN - 8755-6863

VL - 46

SP - 179

EP - 183

JO - Pediatric Pulmonology

JF - Pediatric Pulmonology

IS - 2

ER -

A bedside assay to detect streptococcus pneumoniae in children with empyema

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