A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting

Sarah M. Hicks; Kai Pohl; Teresa Neeman; Hayley A. Mcnamara; Kate M. Parsons; Jin Shu He; Sidra A. Ali; Samina Nazir; Louise C. Rowntree; Thi H.O. Nguyen; Katherine Kedzierska; Denise L. Doolan; Carola G. Vinuesa; Matthew C. Cook; Nicholas Coatsworth; Paul S. Myles; Florian Kurth; Leif E. Sander; Graham J. Mann; Russell L. Gruen; Amee J. George; Elizabeth E. Gardiner; Ian A. Cockburn

doi:10.1093/infdis/jiaa623

A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting

Sarah M. Hicks, Kai Pohl, Teresa Neeman, Hayley A. Mcnamara, Kate M. Parsons, Jin Shu He, Sidra A. Ali, Samina Nazir, Louise C. Rowntree, Thi H.O. Nguyen, Katherine Kedzierska, Denise L. Doolan, Carola G. Vinuesa, Matthew C. Cook, Nicholas Coatsworth, Paul S. Myles, Florian Kurth, Leif E. Sander, Graham J. Mann, Russell L. GruenAmee J. George, Elizabeth E. Gardiner, Ian A. Cockburn^*

^*Corresponding author for this work

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16 Citations (Scopus)

Abstract

Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.

Original language	English
Pages (from-to)	10-14
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	223
Issue number	1
DOIs	https://doi.org/10.1093/infdis/jiaa623
Publication status	Published - 1 Jan 2021

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Hicks, S. M., Pohl, K., Neeman, T., Mcnamara, H. A., Parsons, K. M., He, J. S., Ali, S. A., Nazir, S., Rowntree, L. C., Nguyen, T. H. O., Kedzierska, K., Doolan, D. L., Vinuesa, C. G., Cook, M. C., Coatsworth, N., Myles, P. S., Kurth, F., Sander, L. E., Mann, G. J., ... Cockburn, I. A. (2021). A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting. Journal of Infectious Diseases, 223(1), 10-14. https://doi.org/10.1093/infdis/jiaa623

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title = "A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting",

abstract = "Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.",

keywords = "COVID-19, ELISA, SARS-CoV-2, antibodies, seroprevalence",

author = "Hicks, {Sarah M.} and Kai Pohl and Teresa Neeman and Mcnamara, {Hayley A.} and Parsons, {Kate M.} and He, {Jin Shu} and Ali, {Sidra A.} and Samina Nazir and Rowntree, {Louise C.} and Nguyen, {Thi H.O.} and Katherine Kedzierska and Doolan, {Denise L.} and Vinuesa, {Carola G.} and Cook, {Matthew C.} and Nicholas Coatsworth and Myles, {Paul S.} and Florian Kurth and Sander, {Leif E.} and Mann, {Graham J.} and Gruen, {Russell L.} and George, {Amee J.} and Gardiner, {Elizabeth E.} and Cockburn, {Ian A.}",

year = "2021",

month = jan,

day = "1",

doi = "10.1093/infdis/jiaa623",

language = "English",

volume = "223",

pages = "10--14",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press ",

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Hicks, SM, Pohl, K, Neeman, T, Mcnamara, HA, Parsons, KM, He, JS, Ali, SA, Nazir, S, Rowntree, LC, Nguyen, THO, Kedzierska, K, Doolan, DL, Vinuesa, CG, Cook, MC, Coatsworth, N, Myles, PS, Kurth, F, Sander, LE, Mann, GJ , Gruen, RL , George, AJ , Gardiner, EE & Cockburn, IA 2021, 'A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting', Journal of Infectious Diseases, vol. 223, no. 1, pp. 10-14. https://doi.org/10.1093/infdis/jiaa623

A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting. / Hicks, Sarah M.; Pohl, Kai; Neeman, Teresa et al.
In: Journal of Infectious Diseases, Vol. 223, No. 1, 01.01.2021, p. 10-14.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting

AU - Hicks, Sarah M.

AU - Pohl, Kai

AU - Neeman, Teresa

AU - Mcnamara, Hayley A.

AU - Parsons, Kate M.

AU - He, Jin Shu

AU - Ali, Sidra A.

AU - Nazir, Samina

AU - Rowntree, Louise C.

AU - Nguyen, Thi H.O.

AU - Kedzierska, Katherine

AU - Doolan, Denise L.

AU - Vinuesa, Carola G.

AU - Cook, Matthew C.

AU - Coatsworth, Nicholas

AU - Myles, Paul S.

AU - Kurth, Florian

AU - Sander, Leif E.

AU - Mann, Graham J.

AU - Gruen, Russell L.

AU - George, Amee J.

AU - Gardiner, Elizabeth E.

AU - Cockburn, Ian A.

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.

AB - Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.

KW - COVID-19

KW - ELISA

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KW - antibodies

KW - seroprevalence

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U2 - 10.1093/infdis/jiaa623

DO - 10.1093/infdis/jiaa623

M3 - Article

SN - 0022-1899

VL - 223

SP - 10

EP - 14

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 1

ER -

A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting

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