A general target selection method for crystallographic proteomics.

Gautier Robin; Nathan P. Cowieson; Gregor Guncar; Jade K. Forwood; Pawel Listwan; David A. Hume; Bostjan Kobe; Jennifer L. Martin; Thomas Huber

doi:10.1007/978-1-60327-058-8_2

A general target selection method for crystallographic proteomics.

Gautier Robin^*, Nathan P. Cowieson, Gregor Guncar, Jade K. Forwood, Pawel Listwan, David A. Hume, Bostjan Kobe, Jennifer L. Martin, Thomas Huber

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Increasing the success in obtaining structures and maximizing the value of the structures determined are the two major goals of target selection in structural proteomics. This chapter presents an efficient and flexible target selection procedure supplemented with a Web-based resource that is suitable for small- to large-scale structural genomics projects that use crystallography as the major means of structure determination. Based on three criteria, biological significance, structural novelty, and "crystallizability," the approach first removes (filters) targets that do not meet minimal criteria and then ranks the remaining targets based on their "crystallizability" estimates. This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects.

Original language	English
Pages (from-to)	27-35
Number of pages	9
Journal	Methods in Molecular Biology
Volume	426
DOIs	https://doi.org/10.1007/978-1-60327-058-8_2
Publication status	Published - 2008
Externally published	Yes

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AU - Robin, Gautier

AU - Cowieson, Nathan P.

AU - Guncar, Gregor

AU - Forwood, Jade K.

AU - Listwan, Pawel

AU - Hume, David A.

AU - Kobe, Bostjan

AU - Martin, Jennifer L.

AU - Huber, Thomas

PY - 2008

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AB - Increasing the success in obtaining structures and maximizing the value of the structures determined are the two major goals of target selection in structural proteomics. This chapter presents an efficient and flexible target selection procedure supplemented with a Web-based resource that is suitable for small- to large-scale structural genomics projects that use crystallography as the major means of structure determination. Based on three criteria, biological significance, structural novelty, and "crystallizability," the approach first removes (filters) targets that do not meet minimal criteria and then ranks the remaining targets based on their "crystallizability" estimates. This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects.

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SN - 1064-3745

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A general target selection method for crystallographic proteomics.

Abstract

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