A high-sensitivity HPLC assay for measuring intracellular Na+ and K+ and its application to Plasmodium falciparum infected erythrocytes

Markus Winterberg; Kiaran Kirk

doi:10.1038/srep29241

A high-sensitivity HPLC assay for measuring intracellular Na⁺ and K⁺and its application to Plasmodium falciparum infected erythrocytes

Markus Winterberg, Kiaran Kirk^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques. Here we present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na + and K + content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609.

Original language	English
Article number	29241
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep29241
Publication status	Published - 7 Jul 2016

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title = "A high-sensitivity HPLC assay for measuring intracellular Na+ and K+ and its application to Plasmodium falciparum infected erythrocytes",

abstract = "The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques. Here we present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na + and K + content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609.",

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AU - Kirk, Kiaran

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N2 - The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques. Here we present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na + and K + content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609.

AB - The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques. Here we present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na + and K + content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609.

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VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 29241

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A high-sensitivity HPLC assay for measuring intracellular Na⁺ and K⁺and its application to Plasmodium falciparum infected erythrocytes

Abstract

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