A lanthanide tag for a complementary set of pseudocontact shifts

Lydia Topping; Adarshi P. Welegedara; Martyna Judd; Elwy H. Abdelkader; Nicholas Cox; Gottfried Otting; Stephen J. Butler

doi:10.1039/d4cc03007a

A lanthanide tag for a complementary set of pseudocontact shifts

Lydia Topping, Adarshi P. Welegedara, Martyna Judd, Elwy H. Abdelkader, Nicholas Cox, Gottfried Otting, Stephen J. Butler^*

^*Corresponding author for this work

Research School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide tag that generates different PCSs than that of a related tag, which differs in structure by a single oxygen atom. It is highly reactive towards cysteine and performs well in turn-on luminescence and in EPR spectroscopy.We present a new lanthanide tag C14, which differs from our C12 tag by a single oxygen atom and generates distinct and complementary pseudocontact shifts, enhancing protein structure determination via paramagnetic NMR, EPR and emission spectroscopy.

Original language	English
Pages (from-to)	8458-8461
Number of pages	4
Journal	Chemical Communications
Volume	60
Issue number	64
DOIs	https://doi.org/10.1039/d4cc03007a
Publication status	Published - 16 Jul 2024

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10.1039/d4cc03007aLicence: CC BY

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abstract = "Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide tag that generates different PCSs than that of a related tag, which differs in structure by a single oxygen atom. It is highly reactive towards cysteine and performs well in turn-on luminescence and in EPR spectroscopy.We present a new lanthanide tag C14, which differs from our C12 tag by a single oxygen atom and generates distinct and complementary pseudocontact shifts, enhancing protein structure determination via paramagnetic NMR, EPR and emission spectroscopy.",

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AU - Topping, Lydia

AU - Welegedara, Adarshi P.

AU - Judd, Martyna

AU - Abdelkader, Elwy H.

AU - Cox, Nicholas

AU - Otting, Gottfried

AU - Butler, Stephen J.

PY - 2024/7/16

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N2 - Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide tag that generates different PCSs than that of a related tag, which differs in structure by a single oxygen atom. It is highly reactive towards cysteine and performs well in turn-on luminescence and in EPR spectroscopy.We present a new lanthanide tag C14, which differs from our C12 tag by a single oxygen atom and generates distinct and complementary pseudocontact shifts, enhancing protein structure determination via paramagnetic NMR, EPR and emission spectroscopy.

AB - Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide tag that generates different PCSs than that of a related tag, which differs in structure by a single oxygen atom. It is highly reactive towards cysteine and performs well in turn-on luminescence and in EPR spectroscopy.We present a new lanthanide tag C14, which differs from our C12 tag by a single oxygen atom and generates distinct and complementary pseudocontact shifts, enhancing protein structure determination via paramagnetic NMR, EPR and emission spectroscopy.

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VL - 60

SP - 8458

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JO - Chemical Communications

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A lanthanide tag for a complementary set of pseudocontact shifts

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