A new and rapid method of making permanent preparations of large numbers of house-dust mites for light microscopy

M. J. Colloff

doi:10.1007/BF01197926

A new and rapid method of making permanent preparations of large numbers of house-dust mites for light microscopy

M. J. Colloff^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A method is described for the transfer to microslides of large numbers of mites extracted from house dust, in a single operation. Mites were frozen in water in a watch glass at -20°C. The ice was then everted onto a siliconeized microslide and the meltwater evaporated off on a hotplate at 55°C. Mounting medium and coverslip were then added in the normal way. The technique avoids the difficulty and tedium of transferring individual mites to a microslide and the haphzard and messy process of transfer in liquid medium via a pipette.

Original language	English
Pages (from-to)	323-326
Number of pages	4
Journal	Experimental & Applied Acarology
Volume	7
Issue number	4
DOIs	https://doi.org/10.1007/BF01197926
Publication status	Published - Sept 1989
Externally published	Yes

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10.1007/BF01197926

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abstract = "A method is described for the transfer to microslides of large numbers of mites extracted from house dust, in a single operation. Mites were frozen in water in a watch glass at -20°C. The ice was then everted onto a siliconeized microslide and the meltwater evaporated off on a hotplate at 55°C. Mounting medium and coverslip were then added in the normal way. The technique avoids the difficulty and tedium of transferring individual mites to a microslide and the haphzard and messy process of transfer in liquid medium via a pipette.",

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AB - A method is described for the transfer to microslides of large numbers of mites extracted from house dust, in a single operation. Mites were frozen in water in a watch glass at -20°C. The ice was then everted onto a siliconeized microslide and the meltwater evaporated off on a hotplate at 55°C. Mounting medium and coverslip were then added in the normal way. The technique avoids the difficulty and tedium of transferring individual mites to a microslide and the haphzard and messy process of transfer in liquid medium via a pipette.

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A new and rapid method of making permanent preparations of large numbers of house-dust mites for light microscopy

Abstract

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