A new approach for spot detection in total internal reflection fluorescence microscopy

Seyed Hamid Rezatofighi; Richard Hartley; William E. Hughes

doi:10.1109/ISBI.2012.6235684

A new approach for spot detection in total internal reflection fluorescence microscopy

Seyed Hamid Rezatofighi^*, Richard Hartley, William E. Hughes

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

22 Citations (Scopus)

Abstract

Biological images acquired from fluorescence microscopy-based imaging techniques, such as total internal reflection fluorescence microscopy, generally contain hundreds of subcellular components, appearing in the images as bright spots. Therefore, the first step of analysis of these images usually involves the detection of these bright spots. In this paper, we propose a new approach for spot detection using the h-dome transform along with an adaptive mask obtained from regional information. Moreover, local gradient information is used in order to distinguish the spots from other structures. To evaluate the performance of our algorithm, we test it on synthetic images and also real TIRFM images and compare our results with those of two recent methods.

Original language	English
Title of host publication	2012 9th IEEE International Symposium on Biomedical Imaging
Subtitle of host publication	From Nano to Macro, ISBI 2012 - Proceedings
Pages	860-863
Number of pages	4
DOIs	https://doi.org/10.1109/ISBI.2012.6235684
Publication status	Published - 2012
Event	2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Barcelona, Spain Duration: 2 May 2012 → 5 May 2012

Publication series

Name	Proceedings - International Symposium on Biomedical Imaging
ISSN (Print)	1945-7928
ISSN (Electronic)	1945-8452

Conference

Conference	2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012
Country/Territory	Spain
City	Barcelona
Period	2/05/12 → 5/05/12

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10.1109/ISBI.2012.6235684

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Rezatofighi, S. H., Hartley, R., & Hughes, W. E. (2012). A new approach for spot detection in total internal reflection fluorescence microscopy. In 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Proceedings (pp. 860-863). Article 6235684 (Proceedings - International Symposium on Biomedical Imaging). https://doi.org/10.1109/ISBI.2012.6235684

@inproceedings{f797f72985704547a0cb77196c13911f,

title = "A new approach for spot detection in total internal reflection fluorescence microscopy",

abstract = "Biological images acquired from fluorescence microscopy-based imaging techniques, such as total internal reflection fluorescence microscopy, generally contain hundreds of subcellular components, appearing in the images as bright spots. Therefore, the first step of analysis of these images usually involves the detection of these bright spots. In this paper, we propose a new approach for spot detection using the h-dome transform along with an adaptive mask obtained from regional information. Moreover, local gradient information is used in order to distinguish the spots from other structures. To evaluate the performance of our algorithm, we test it on synthetic images and also real TIRFM images and compare our results with those of two recent methods.",

keywords = "Spot detection, TIRFM, gray-scale morphology, h-dome transform",

author = "Rezatofighi, {Seyed Hamid} and Richard Hartley and Hughes, {William E.}",

year = "2012",

doi = "10.1109/ISBI.2012.6235684",

language = "English",

isbn = "9781457718588",

series = "Proceedings - International Symposium on Biomedical Imaging",

pages = "860--863",

booktitle = "2012 9th IEEE International Symposium on Biomedical Imaging",

note = "2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 ; Conference date: 02-05-2012 Through 05-05-2012",

}

Rezatofighi, SH, Hartley, R & Hughes, WE 2012, A new approach for spot detection in total internal reflection fluorescence microscopy. in 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Proceedings., 6235684, Proceedings - International Symposium on Biomedical Imaging, pp. 860-863, 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012, Barcelona, Spain, 2/05/12. https://doi.org/10.1109/ISBI.2012.6235684

A new approach for spot detection in total internal reflection fluorescence microscopy. / Rezatofighi, Seyed Hamid; Hartley, Richard; Hughes, William E.
2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Proceedings. 2012. p. 860-863 6235684 (Proceedings - International Symposium on Biomedical Imaging).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A new approach for spot detection in total internal reflection fluorescence microscopy

AU - Rezatofighi, Seyed Hamid

AU - Hartley, Richard

AU - Hughes, William E.

PY - 2012

Y1 - 2012

N2 - Biological images acquired from fluorescence microscopy-based imaging techniques, such as total internal reflection fluorescence microscopy, generally contain hundreds of subcellular components, appearing in the images as bright spots. Therefore, the first step of analysis of these images usually involves the detection of these bright spots. In this paper, we propose a new approach for spot detection using the h-dome transform along with an adaptive mask obtained from regional information. Moreover, local gradient information is used in order to distinguish the spots from other structures. To evaluate the performance of our algorithm, we test it on synthetic images and also real TIRFM images and compare our results with those of two recent methods.

AB - Biological images acquired from fluorescence microscopy-based imaging techniques, such as total internal reflection fluorescence microscopy, generally contain hundreds of subcellular components, appearing in the images as bright spots. Therefore, the first step of analysis of these images usually involves the detection of these bright spots. In this paper, we propose a new approach for spot detection using the h-dome transform along with an adaptive mask obtained from regional information. Moreover, local gradient information is used in order to distinguish the spots from other structures. To evaluate the performance of our algorithm, we test it on synthetic images and also real TIRFM images and compare our results with those of two recent methods.

KW - Spot detection

KW - TIRFM

KW - gray-scale morphology

KW - h-dome transform

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U2 - 10.1109/ISBI.2012.6235684

DO - 10.1109/ISBI.2012.6235684

M3 - Conference contribution

SN - 9781457718588

T3 - Proceedings - International Symposium on Biomedical Imaging

SP - 860

EP - 863

BT - 2012 9th IEEE International Symposium on Biomedical Imaging

T2 - 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012

Y2 - 2 May 2012 through 5 May 2012

ER -

A new approach for spot detection in total internal reflection fluorescence microscopy

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