A novel binding assay to assess specificity of monoclonal antibodies

Hilary S. Warren*, Jayaram Bettadapura

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)


    Dynabeads® TALON™ are uniform superparamagnetic polystyrene beads of 1μm diameter that bind 6-His-tagged recombinant proteins through Co ++-affinity binding, and are normally used for protein purification. We have used these beads to bind 6-His-rNKp30 and 6-His-rNKp46 to use as a matrix for evaluating NKp30 and NKp46 mAb submitted to the 8th International Human Leukocyte Differentiation Antigen Workshop. We show that recombinant protein coated beads are an effective tool to evaluate the specificity and epitope reactivity of mAb.

    Original languageEnglish
    Pages (from-to)33-38
    Number of pages6
    JournalJournal of Immunological Methods
    Issue number1
    Publication statusPublished - 20 Oct 2005


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