A novel binding assay to assess specificity of monoclonal antibodies

Hilary S. Warren; Jayaram Bettadapura

doi:10.1016/j.jim.2005.07.011

A novel binding assay to assess specificity of monoclonal antibodies

Hilary S. Warren^*, Jayaram Bettadapura

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Dynabeads® TALON™ are uniform superparamagnetic polystyrene beads of 1μm diameter that bind 6-His-tagged recombinant proteins through Co ⁺⁺-affinity binding, and are normally used for protein purification. We have used these beads to bind 6-His-rNKp30 and 6-His-rNKp46 to use as a matrix for evaluating NKp30 and NKp46 mAb submitted to the 8th International Human Leukocyte Differentiation Antigen Workshop. We show that recombinant protein coated beads are an effective tool to evaluate the specificity and epitope reactivity of mAb.

Original language	English
Pages (from-to)	33-38
Number of pages	6
Journal	Journal of Immunological Methods
Volume	305
Issue number	1
DOIs	https://doi.org/10.1016/j.jim.2005.07.011
Publication status	Published - 20 Oct 2005

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10.1016/j.jim.2005.07.011

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title = "A novel binding assay to assess specificity of monoclonal antibodies",

abstract = "Dynabeads{\textregistered} TALON{\texttrademark} are uniform superparamagnetic polystyrene beads of 1μm diameter that bind 6-His-tagged recombinant proteins through Co ++-affinity binding, and are normally used for protein purification. We have used these beads to bind 6-His-rNKp30 and 6-His-rNKp46 to use as a matrix for evaluating NKp30 and NKp46 mAb submitted to the 8th International Human Leukocyte Differentiation Antigen Workshop. We show that recombinant protein coated beads are an effective tool to evaluate the specificity and epitope reactivity of mAb.",

keywords = "6-His recombinant protein, Magnetic beads, Monoclonal antibody, NK cells, NKp30, NKp46",

author = "Warren, {Hilary S.} and Jayaram Bettadapura",

year = "2005",

month = oct,

day = "20",

doi = "10.1016/j.jim.2005.07.011",

language = "English",

volume = "305",

pages = "33--38",

journal = "Journal of Immunological Methods",

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T1 - A novel binding assay to assess specificity of monoclonal antibodies

AU - Warren, Hilary S.

AU - Bettadapura, Jayaram

PY - 2005/10/20

Y1 - 2005/10/20

N2 - Dynabeads® TALON™ are uniform superparamagnetic polystyrene beads of 1μm diameter that bind 6-His-tagged recombinant proteins through Co ++-affinity binding, and are normally used for protein purification. We have used these beads to bind 6-His-rNKp30 and 6-His-rNKp46 to use as a matrix for evaluating NKp30 and NKp46 mAb submitted to the 8th International Human Leukocyte Differentiation Antigen Workshop. We show that recombinant protein coated beads are an effective tool to evaluate the specificity and epitope reactivity of mAb.

AB - Dynabeads® TALON™ are uniform superparamagnetic polystyrene beads of 1μm diameter that bind 6-His-tagged recombinant proteins through Co ++-affinity binding, and are normally used for protein purification. We have used these beads to bind 6-His-rNKp30 and 6-His-rNKp46 to use as a matrix for evaluating NKp30 and NKp46 mAb submitted to the 8th International Human Leukocyte Differentiation Antigen Workshop. We show that recombinant protein coated beads are an effective tool to evaluate the specificity and epitope reactivity of mAb.

KW - 6-His recombinant protein

KW - Magnetic beads

KW - Monoclonal antibody

KW - NK cells

KW - NKp30

KW - NKp46

UR - http://www.scopus.com/inward/record.url?scp=26244437476&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2005.07.011

DO - 10.1016/j.jim.2005.07.011

M3 - Article

SN - 0022-1759

VL - 305

SP - 33

EP - 38

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1

ER -

A novel binding assay to assess specificity of monoclonal antibodies

Abstract

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