A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity

François Xavier Gallat; Alex P.S. Brogan; Yann Fichou; Nina McGrath; Martine Moulin; Michael Härtlein; Jérôme Combet; Joachim Wuttke; Stephen Mann; Giuseppe Zaccai; Colin J. Jackson; Adam W. Perriman; Martin Weik

doi:10.1021/ja303894g

A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity

François Xavier Gallat, Alex P.S. Brogan, Yann Fichou, Nina McGrath, Martine Moulin, Michael Härtlein, Jérôme Combet, Joachim Wuttke, Stephen Mann, Giuseppe Zaccai, Colin J. Jackson, Adam W. Perriman^*, Martin Weik

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

The observation of biological activity in solvent-free protein-polymer surfactant hybrids challenges the view of aqueous and nonaqueous solvents being unique promoters of protein dynamics linked to function. Here, we combine elastic incoherent neutron scattering and specific deuterium labeling to separately study protein and polymer motions in solvent-free hybrids. Myoglobin motions within the hybrid are found to closely resemble those of a hydrated protein, and motions of the polymer surfactant coating are similar to those of the hydration water, leading to the conclusion that the polymer surfactant coating plasticizes protein structures in a way similar to hydration water.

Original language	English
Pages (from-to)	13168-13171
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	134
Issue number	32
DOIs	https://doi.org/10.1021/ja303894g
Publication status	Published - 15 Aug 2012

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Gallat, F. X., Brogan, A. P. S., Fichou, Y., McGrath, N., Moulin, M., Härtlein, M., Combet, J., Wuttke, J., Mann, S., Zaccai, G., Jackson, C. J., Perriman, A. W., & Weik, M. (2012). A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity. Journal of the American Chemical Society, 134(32), 13168-13171. https://doi.org/10.1021/ja303894g

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title = "A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity",

abstract = "The observation of biological activity in solvent-free protein-polymer surfactant hybrids challenges the view of aqueous and nonaqueous solvents being unique promoters of protein dynamics linked to function. Here, we combine elastic incoherent neutron scattering and specific deuterium labeling to separately study protein and polymer motions in solvent-free hybrids. Myoglobin motions within the hybrid are found to closely resemble those of a hydrated protein, and motions of the polymer surfactant coating are similar to those of the hydration water, leading to the conclusion that the polymer surfactant coating plasticizes protein structures in a way similar to hydration water.",

author = "Gallat, {Fran{\c c}ois Xavier} and Brogan, {Alex P.S.} and Yann Fichou and Nina McGrath and Martine Moulin and Michael H{\"a}rtlein and J{\'e}r{\^o}me Combet and Joachim Wuttke and Stephen Mann and Giuseppe Zaccai and Jackson, {Colin J.} and Perriman, {Adam W.} and Martin Weik",

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Gallat, FX, Brogan, APS, Fichou, Y, McGrath, N, Moulin, M, Härtlein, M, Combet, J, Wuttke, J, Mann, S, Zaccai, G, Jackson, CJ , Perriman, AW & Weik, M 2012, 'A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity', Journal of the American Chemical Society, vol. 134, no. 32, pp. 13168-13171. https://doi.org/10.1021/ja303894g

TY - JOUR

T1 - A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity

AU - Gallat, François Xavier

AU - Brogan, Alex P.S.

AU - Fichou, Yann

AU - McGrath, Nina

AU - Moulin, Martine

AU - Härtlein, Michael

AU - Combet, Jérôme

AU - Wuttke, Joachim

AU - Mann, Stephen

AU - Zaccai, Giuseppe

AU - Jackson, Colin J.

AU - Perriman, Adam W.

AU - Weik, Martin

PY - 2012/8/15

Y1 - 2012/8/15

N2 - The observation of biological activity in solvent-free protein-polymer surfactant hybrids challenges the view of aqueous and nonaqueous solvents being unique promoters of protein dynamics linked to function. Here, we combine elastic incoherent neutron scattering and specific deuterium labeling to separately study protein and polymer motions in solvent-free hybrids. Myoglobin motions within the hybrid are found to closely resemble those of a hydrated protein, and motions of the polymer surfactant coating are similar to those of the hydration water, leading to the conclusion that the polymer surfactant coating plasticizes protein structures in a way similar to hydration water.

AB - The observation of biological activity in solvent-free protein-polymer surfactant hybrids challenges the view of aqueous and nonaqueous solvents being unique promoters of protein dynamics linked to function. Here, we combine elastic incoherent neutron scattering and specific deuterium labeling to separately study protein and polymer motions in solvent-free hybrids. Myoglobin motions within the hybrid are found to closely resemble those of a hydrated protein, and motions of the polymer surfactant coating are similar to those of the hydration water, leading to the conclusion that the polymer surfactant coating plasticizes protein structures in a way similar to hydration water.

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U2 - 10.1021/ja303894g

DO - 10.1021/ja303894g

M3 - Article

SN - 0002-7863

VL - 134

SP - 13168

EP - 13171

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 32

ER -

A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity

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