A protocol to evaluate immunoglobulin deposits in mouse glomeruli

Sonia B. Nambadan; Maurice Stanley; Yaoyuan Zhang; Vicki Athanasopoulos; Simon H. Jiang

doi:10.1016/j.xpro.2022.101375

A protocol to evaluate immunoglobulin deposits in mouse glomeruli

Sonia B. Nambadan, Maurice Stanley, Yaoyuan Zhang, Vicki Athanasopoulos, Simon H. Jiang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Accumulation of immunoglobulin and complement components within the kidneys is a hallmark of glomerulonephritis. Staining and detection of IgG, IgA, IgM, and C3 deposits can assist in diagnosing the underlying causes of nephritis and has implications for the pathological processes underpinning glomerulonephritis. Here, we describe a protocol to detect immune deposits within biological specimens such as mouse kidneys. We detail tissue isolation and processing, immunostaining, and fluorescence microscopy to characterize and quantify the extent of immunological deposits contributing to kidney injury. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).

Original language	English
Article number	101375
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101375
Publication status	Published - 17 Jun 2022

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title = "A protocol to evaluate immunoglobulin deposits in mouse glomeruli",

abstract = "Accumulation of immunoglobulin and complement components within the kidneys is a hallmark of glomerulonephritis. Staining and detection of IgG, IgA, IgM, and C3 deposits can assist in diagnosing the underlying causes of nephritis and has implications for the pathological processes underpinning glomerulonephritis. Here, we describe a protocol to detect immune deposits within biological specimens such as mouse kidneys. We detail tissue isolation and processing, immunostaining, and fluorescence microscopy to characterize and quantify the extent of immunological deposits contributing to kidney injury. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).",

keywords = "Immunology, Microscopy, Model Organisms",

author = "Nambadan, {Sonia B.} and Maurice Stanley and Yaoyuan Zhang and Vicki Athanasopoulos and Jiang, {Simon H.}",

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year = "2022",

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T1 - A protocol to evaluate immunoglobulin deposits in mouse glomeruli

AU - Nambadan, Sonia B.

AU - Stanley, Maurice

AU - Zhang, Yaoyuan

AU - Athanasopoulos, Vicki

AU - Jiang, Simon H.

PY - 2022/6/17

Y1 - 2022/6/17

N2 - Accumulation of immunoglobulin and complement components within the kidneys is a hallmark of glomerulonephritis. Staining and detection of IgG, IgA, IgM, and C3 deposits can assist in diagnosing the underlying causes of nephritis and has implications for the pathological processes underpinning glomerulonephritis. Here, we describe a protocol to detect immune deposits within biological specimens such as mouse kidneys. We detail tissue isolation and processing, immunostaining, and fluorescence microscopy to characterize and quantify the extent of immunological deposits contributing to kidney injury. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).

AB - Accumulation of immunoglobulin and complement components within the kidneys is a hallmark of glomerulonephritis. Staining and detection of IgG, IgA, IgM, and C3 deposits can assist in diagnosing the underlying causes of nephritis and has implications for the pathological processes underpinning glomerulonephritis. Here, we describe a protocol to detect immune deposits within biological specimens such as mouse kidneys. We detail tissue isolation and processing, immunostaining, and fluorescence microscopy to characterize and quantify the extent of immunological deposits contributing to kidney injury. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).

KW - Immunology

KW - Microscopy

KW - Model Organisms

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DO - 10.1016/j.xpro.2022.101375

M3 - Article

SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 101375

ER -

A protocol to evaluate immunoglobulin deposits in mouse glomeruli

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