A quantitative assessment of the carbonic anhydrase activity in photosystem II

I. L. McConnell; M. R. Badger; T. Wydrzynski; W. Hillier

doi:10.1016/j.bbabio.2007.01.019

A quantitative assessment of the carbonic anhydrase activity in photosystem II

I. L. McConnell^*, M. R. Badger, T. Wydrzynski, W. Hillier

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Using a carbonic anhydrase assay based on membrane inlet mass spectrometry (MIMS), we have extended our earlier investigations of Photosystem II (PSII)-associated carbonic anhydrase activity in spinach PSII preparations (W. Hillier, I. McConnell, M. R. Badger, A. Boussac, V.V. Klimov G. C. Dismukes, T. Wydrzynski Biochemistry 2006, 45:2094). The relationship between the carbonic anhydrase activity and O₂ evolution has been evaluated in terms of the effects of metal ion addition, preparation type, light, and response to specific inhibitors. The results indicate that the PSII-associated carbonic anhydrase activity is variable and appears not to be associated specifically with the oxygen evolving activity nor the 33 kDa extrinsic manganese stabilising protein.

Original language	English
Pages (from-to)	639-647
Number of pages	9
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1767
Issue number	6
DOIs	https://doi.org/10.1016/j.bbabio.2007.01.019
Publication status	Published - Jun 2007

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title = "A quantitative assessment of the carbonic anhydrase activity in photosystem II",

abstract = "Using a carbonic anhydrase assay based on membrane inlet mass spectrometry (MIMS), we have extended our earlier investigations of Photosystem II (PSII)-associated carbonic anhydrase activity in spinach PSII preparations (W. Hillier, I. McConnell, M. R. Badger, A. Boussac, V.V. Klimov G. C. Dismukes, T. Wydrzynski Biochemistry 2006, 45:2094). The relationship between the carbonic anhydrase activity and O2 evolution has been evaluated in terms of the effects of metal ion addition, preparation type, light, and response to specific inhibitors. The results indicate that the PSII-associated carbonic anhydrase activity is variable and appears not to be associated specifically with the oxygen evolving activity nor the 33 kDa extrinsic manganese stabilising protein.",

keywords = "Bicarbonate, Carbonic anhydrase, Manganese stabilising protein, Oxygen evolving complex, Photosystem II",

author = "McConnell, {I. L.} and Badger, {M. R.} and T. Wydrzynski and W. Hillier",

year = "2007",

month = jun,

doi = "10.1016/j.bbabio.2007.01.019",

language = "English",

volume = "1767",

pages = "639--647",

journal = "Biochimica et Biophysica Acta - Bioenergetics",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - A quantitative assessment of the carbonic anhydrase activity in photosystem II

AU - McConnell, I. L.

AU - Badger, M. R.

AU - Wydrzynski, T.

AU - Hillier, W.

PY - 2007/6

Y1 - 2007/6

N2 - Using a carbonic anhydrase assay based on membrane inlet mass spectrometry (MIMS), we have extended our earlier investigations of Photosystem II (PSII)-associated carbonic anhydrase activity in spinach PSII preparations (W. Hillier, I. McConnell, M. R. Badger, A. Boussac, V.V. Klimov G. C. Dismukes, T. Wydrzynski Biochemistry 2006, 45:2094). The relationship between the carbonic anhydrase activity and O2 evolution has been evaluated in terms of the effects of metal ion addition, preparation type, light, and response to specific inhibitors. The results indicate that the PSII-associated carbonic anhydrase activity is variable and appears not to be associated specifically with the oxygen evolving activity nor the 33 kDa extrinsic manganese stabilising protein.

AB - Using a carbonic anhydrase assay based on membrane inlet mass spectrometry (MIMS), we have extended our earlier investigations of Photosystem II (PSII)-associated carbonic anhydrase activity in spinach PSII preparations (W. Hillier, I. McConnell, M. R. Badger, A. Boussac, V.V. Klimov G. C. Dismukes, T. Wydrzynski Biochemistry 2006, 45:2094). The relationship between the carbonic anhydrase activity and O2 evolution has been evaluated in terms of the effects of metal ion addition, preparation type, light, and response to specific inhibitors. The results indicate that the PSII-associated carbonic anhydrase activity is variable and appears not to be associated specifically with the oxygen evolving activity nor the 33 kDa extrinsic manganese stabilising protein.

KW - Bicarbonate

KW - Carbonic anhydrase

KW - Manganese stabilising protein

KW - Oxygen evolving complex

KW - Photosystem II

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U2 - 10.1016/j.bbabio.2007.01.019

DO - 10.1016/j.bbabio.2007.01.019

M3 - Article

SN - 0005-2728

VL - 1767

SP - 639

EP - 647

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

IS - 6

ER -

A quantitative assessment of the carbonic anhydrase activity in photosystem II

Abstract

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