A rapid and accurate bioluminescence-based migration assay permitting analysis of tumor cell/stromal cell interactions

Jinsoo Yoon; Christopher R. Parish; Lucy A. Coupland

doi:10.3390/mps3010010

A rapid and accurate bioluminescence-based migration assay permitting analysis of tumor cell/stromal cell interactions

Jinsoo Yoon, Christopher R. Parish, Lucy A. Coupland^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Bioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor cells with different stromal cell types for analysis of the effects of intercellular cross-talk on tumor cell migration. We describe a novel migration assay using bioluminescent tumor cell lines that is rapid, accurate, and permits the study of the effects of tumor cell-stromal cell interactions on tumor cell migratory behavior.

Original language	English
Article number	10
Pages (from-to)	1-12
Number of pages	12
Journal	Methods and Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.3390/mps3010010
Publication status	Published - 2020

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10.3390/mps3010010

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title = "A rapid and accurate bioluminescence-based migration assay permitting analysis of tumor cell/stromal cell interactions",

abstract = "Bioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor cells with different stromal cell types for analysis of the effects of intercellular cross-talk on tumor cell migration. We describe a novel migration assay using bioluminescent tumor cell lines that is rapid, accurate, and permits the study of the effects of tumor cell-stromal cell interactions on tumor cell migratory behavior.",

keywords = "Bioluminescence, Transwell migration assay, Tumor/stromal interactions",

author = "Jinsoo Yoon and Parish, {Christopher R.} and Coupland, {Lucy A.}",

note = "Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

doi = "10.3390/mps3010010",

language = "English",

volume = "3",

pages = "1--12",

journal = "Methods and Protocols",

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TY - JOUR

T1 - A rapid and accurate bioluminescence-based migration assay permitting analysis of tumor cell/stromal cell interactions

AU - Yoon, Jinsoo

AU - Parish, Christopher R.

AU - Coupland, Lucy A.

PY - 2020

Y1 - 2020

N2 - Bioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor cells with different stromal cell types for analysis of the effects of intercellular cross-talk on tumor cell migration. We describe a novel migration assay using bioluminescent tumor cell lines that is rapid, accurate, and permits the study of the effects of tumor cell-stromal cell interactions on tumor cell migratory behavior.

AB - Bioluminescent tumor cell lines are used extensively in vivo to monitor tumor growth and metastasis but rarely used in vitro to follow tumor cell behavior. Tumor cell migration is frequently studied in vitro using transwell assays, however, current methods do not permit the co-incubation of tumor cells with different stromal cell types for analysis of the effects of intercellular cross-talk on tumor cell migration. We describe a novel migration assay using bioluminescent tumor cell lines that is rapid, accurate, and permits the study of the effects of tumor cell-stromal cell interactions on tumor cell migratory behavior.

KW - Bioluminescence

KW - Transwell migration assay

KW - Tumor/stromal interactions

UR - http://www.scopus.com/inward/record.url?scp=85089847268&partnerID=8YFLogxK

U2 - 10.3390/mps3010010

DO - 10.3390/mps3010010

M3 - Article

SN - 2409-9279

VL - 3

SP - 1

EP - 12

JO - Methods and Protocols

JF - Methods and Protocols

IS - 1

M1 - 10

ER -

A rapid and accurate bioluminescence-based migration assay permitting analysis of tumor cell/stromal cell interactions

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