A recombinant avipoxvirus HIV-1 vaccine expressing interferon-gamma is safe and immunogenic in macaques

Stephen J. Kent; Anne Zhao; C. Jane Dale; Sally Land; David B. Boyle; Ian A. Ramshaw

doi:10.1016/S0264-410X(99)00559-9

A recombinant avipoxvirus HIV-1 vaccine expressing interferon-gamma is safe and immunogenic in macaques

Stephen J. Kent^*, Anne Zhao, C. Jane Dale, Sally Land, David B. Boyle, Ian A. Ramshaw

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Complex recombinant fowlpoxvirus (rFPV) vaccines expressing both HIV-1 antigens and type 1 cytokines could facilitate the induction of cellular immunity against HIV-1. A single rFPV expressing both HIV-1gag/pol and human interferon-γ (FPVgag/pol-IFNγ) was constructed and assessed as a therapeutic vaccine for safety and immunogenicity in macaques (Macaca nemestrina) previously infected with HIV-1. FPV gag/pol-IFNγ vaccinations were safe and enhanced T cell proliferative responses to Gag antigens (but not control tetanus antigens). Enhanced CTL responses to gag/pol antigens were also observed following IFNγ expressing vaccinations. Since cellular immunity may be critical to controlling or preventing HIV-1 infection, these observations suggest that avipox vectors co-expressing IFNγ should be further evaluated as therapeutic or preventive HIV-1 vaccines. (C) 2000 Elsevier Science Ltd.

Original language	English
Pages (from-to)	2250-2256
Number of pages	7
Journal	Vaccine
Volume	18
Issue number	21
DOIs	https://doi.org/10.1016/S0264-410X(99)00559-9
Publication status	Published - Apr 2000

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title = "A recombinant avipoxvirus HIV-1 vaccine expressing interferon-gamma is safe and immunogenic in macaques",

abstract = "Complex recombinant fowlpoxvirus (rFPV) vaccines expressing both HIV-1 antigens and type 1 cytokines could facilitate the induction of cellular immunity against HIV-1. A single rFPV expressing both HIV-1gag/pol and human interferon-γ (FPVgag/pol-IFNγ) was constructed and assessed as a therapeutic vaccine for safety and immunogenicity in macaques (Macaca nemestrina) previously infected with HIV-1. FPV gag/pol-IFNγ vaccinations were safe and enhanced T cell proliferative responses to Gag antigens (but not control tetanus antigens). Enhanced CTL responses to gag/pol antigens were also observed following IFNγ expressing vaccinations. Since cellular immunity may be critical to controlling or preventing HIV-1 infection, these observations suggest that avipox vectors co-expressing IFNγ should be further evaluated as therapeutic or preventive HIV-1 vaccines. (C) 2000 Elsevier Science Ltd.",

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AU - Dale, C. Jane

AU - Land, Sally

AU - Boyle, David B.

AU - Ramshaw, Ian A.

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AB - Complex recombinant fowlpoxvirus (rFPV) vaccines expressing both HIV-1 antigens and type 1 cytokines could facilitate the induction of cellular immunity against HIV-1. A single rFPV expressing both HIV-1gag/pol and human interferon-γ (FPVgag/pol-IFNγ) was constructed and assessed as a therapeutic vaccine for safety and immunogenicity in macaques (Macaca nemestrina) previously infected with HIV-1. FPV gag/pol-IFNγ vaccinations were safe and enhanced T cell proliferative responses to Gag antigens (but not control tetanus antigens). Enhanced CTL responses to gag/pol antigens were also observed following IFNγ expressing vaccinations. Since cellular immunity may be critical to controlling or preventing HIV-1 infection, these observations suggest that avipox vectors co-expressing IFNγ should be further evaluated as therapeutic or preventive HIV-1 vaccines. (C) 2000 Elsevier Science Ltd.

KW - Cellular immunity

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A recombinant avipoxvirus HIV-1 vaccine expressing interferon-gamma is safe and immunogenic in macaques

Abstract

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