A role for IL-5 in the induction of cytotoxic T lymphocytes in vivo

Vasso Apostolopoulos*, Lan F.C. McKenzie, Catherine Lees, Klaus I. Matthaei, Lan G. Young

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    IL-5 is generally regarded as a Th2 cytokine involved in eosinophil maturation and function and in B cell growth and antibody production, but without any well-established effects on T cells. Early reports suggested that IL-5 could stimulate the production of cytotoxic T lymphocytes (CTL) in vitro, but no evidence has been obtained to date for such a role in studies with IL-deficient (IL-5(-/-)) mice. Here we demonstrate that when oxidized mannan MUC1 fusion protein (M-FP) is used as an antigen in mice, IL-5 is required for the optimal generation of the CTL response. IL-5 was as effective as IL-2 for the induction of CTL from spleen cells in vitro and both CD4+ and CD8+ T cells from M-FP-immunized animals could be shown to secrete IL-5 in culture. In IL-5(-/-) mice, CTLp frequency was greatly diminished resulting in the inability to reject MUC1+ tumors. Clearly, IL-5 is produced by functional T cells, especially the Tc1 type, after M-FP immunization and is required for an optimal CTL response to this antigen.

    Original languageEnglish
    Pages (from-to)1733-1739
    Number of pages7
    JournalEuropean Journal of Immunology
    Volume30
    Issue number6
    DOIs
    Publication statusPublished - 2000

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