A second-generation anchored genetic linkage map of the tammar wallaby (Macropus eugenii)

Chenwei Wang; Lee Webley; Ke Jun Wei; Matthew J. Wakefield; Hardip R. Patel; Janine E. Deakin; Amber Alsop; Jennifer A. Marshall Graves; Desmond W. Cooper; Frank W. Nicholas; Kyall R. Zenger

doi:10.1186/1471-2156-12-72

A second-generation anchored genetic linkage map of the tammar wallaby (Macropus eugenii)

Chenwei Wang^*, Lee Webley, Ke Jun Wei, Matthew J. Wakefield, Hardip R. Patel, Janine E. Deakin, Amber Alsop, Jennifer A. Marshall Graves, Desmond W. Cooper, Frank W. Nicholas, Kyall R. Zenger

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Background: The tammar wallaby, Macropus eugenii, a small kangaroo used for decades for studies of reproduction and metabolism, is the model Australian marsupial for genome sequencing and genetic investigations. The production of a more comprehensive cytogenetically-anchored genetic linkage map will significantly contribute to the deciphering of the tammar wallaby genome. It has great value as a resource to identify novel genes and for comparative studies, and is vital for the ongoing genome sequence assembly and gene ordering in this species.Results: A second-generation anchored tammar wallaby genetic linkage map has been constructed based on a total of 148 loci. The linkage map contains the original 64 loci included in the first-generation map, plus an additional 84 microsatellite loci that were chosen specifically to increase coverage and assist with the anchoring and orientation of linkage groups to chromosomes. These additional loci were derived from (a) sequenced BAC clones that had been previously mapped to tammar wallaby chromosomes by fluorescence in situ hybridization (FISH), (b) End sequence from BACs subsequently FISH-mapped to tammar wallaby chromosomes, and

Original language	English
Article number	72
Journal	BMC Genetics
Volume	12
DOIs	https://doi.org/10.1186/1471-2156-12-72
Publication status	Published - 19 Aug 2011

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title = "A second-generation anchored genetic linkage map of the tammar wallaby (Macropus eugenii)",

abstract = "Background: The tammar wallaby, Macropus eugenii, a small kangaroo used for decades for studies of reproduction and metabolism, is the model Australian marsupial for genome sequencing and genetic investigations. The production of a more comprehensive cytogenetically-anchored genetic linkage map will significantly contribute to the deciphering of the tammar wallaby genome. It has great value as a resource to identify novel genes and for comparative studies, and is vital for the ongoing genome sequence assembly and gene ordering in this species.Results: A second-generation anchored tammar wallaby genetic linkage map has been constructed based on a total of 148 loci. The linkage map contains the original 64 loci included in the first-generation map, plus an additional 84 microsatellite loci that were chosen specifically to increase coverage and assist with the anchoring and orientation of linkage groups to chromosomes. These additional loci were derived from (a) sequenced BAC clones that had been previously mapped to tammar wallaby chromosomes by fluorescence in situ hybridization (FISH), (b) End sequence from BACs subsequently FISH-mapped to tammar wallaby chromosomes, and",

author = "Chenwei Wang and Lee Webley and Wei, {Ke Jun} and Wakefield, {Matthew J.} and Patel, {Hardip R.} and Deakin, {Janine E.} and Amber Alsop and {Marshall Graves}, {Jennifer A.} and Cooper, {Desmond W.} and Nicholas, {Frank W.} and Zenger, {Kyall R.}",

year = "2011",

month = aug,

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doi = "10.1186/1471-2156-12-72",

language = "English",

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AU - Wang, Chenwei

AU - Webley, Lee

AU - Wei, Ke Jun

AU - Wakefield, Matthew J.

AU - Patel, Hardip R.

AU - Deakin, Janine E.

AU - Alsop, Amber

AU - Marshall Graves, Jennifer A.

AU - Cooper, Desmond W.

AU - Nicholas, Frank W.

AU - Zenger, Kyall R.

PY - 2011/8/19

Y1 - 2011/8/19

N2 - Background: The tammar wallaby, Macropus eugenii, a small kangaroo used for decades for studies of reproduction and metabolism, is the model Australian marsupial for genome sequencing and genetic investigations. The production of a more comprehensive cytogenetically-anchored genetic linkage map will significantly contribute to the deciphering of the tammar wallaby genome. It has great value as a resource to identify novel genes and for comparative studies, and is vital for the ongoing genome sequence assembly and gene ordering in this species.Results: A second-generation anchored tammar wallaby genetic linkage map has been constructed based on a total of 148 loci. The linkage map contains the original 64 loci included in the first-generation map, plus an additional 84 microsatellite loci that were chosen specifically to increase coverage and assist with the anchoring and orientation of linkage groups to chromosomes. These additional loci were derived from (a) sequenced BAC clones that had been previously mapped to tammar wallaby chromosomes by fluorescence in situ hybridization (FISH), (b) End sequence from BACs subsequently FISH-mapped to tammar wallaby chromosomes, and

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A second-generation anchored genetic linkage map of the tammar wallaby (Macropus eugenii)

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