A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

Benjamin Schwessinger; Xiang  Li; Thomas L  Ellinghaus; Leanne Jade G. Chan; Tong wei; Anna Joe; Nicholas Thomas; Rory Pruitt; Paul D. Adams; Mawsheng Chern; Christopher J. Petzold; Pamela Ronald

doi:10.1039/c5ib00232j

A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

Benjamin Schwessinger, Xiang Li, Thomas L Ellinghaus, Leanne Jade G. Chan, Tong wei, Anna Joe, Nicholas Thomas, Rory Pruitt, Paul D. Adams, Mawsheng Chern, Christopher J. Petzold, Pamela Ronald

Research output: Contribution to journal › Article › peer-review

Abstract

Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.

Original language	English
Pages (from-to)	542-545
Journal	Integrative Biology
Volume	8
Issue number	4
DOIs	https://doi.org/10.1039/c5ib00232j
Publication status	Published - 2016

Access to Document

10.1039/c5ib00232j

Cite this

@article{42a3098893e2448bad1335774bd5b63e,

title = "A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection",

abstract = "Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.",

author = "Benjamin Schwessinger and Xiang Li and Ellinghaus, {Thomas L} and Chan, {Leanne Jade G.} and Tong wei and Anna Joe and Nicholas Thomas and Rory Pruitt and Adams, {Paul D.} and Mawsheng Chern and Petzold, {Christopher J.} and Pamela Ronald",

year = "2016",

doi = "10.1039/c5ib00232j",

language = "English",

volume = "8",

pages = "542--545",

journal = "Integrative Biology ",

publisher = "Royal Society of Chemistry",

number = "4",

}

TY - JOUR

T1 - A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

AU - Schwessinger, Benjamin

AU - Li, Xiang

AU - Ellinghaus, Thomas L

AU - Chan, Leanne Jade G.

AU - wei, Tong

AU - Joe, Anna

AU - Thomas, Nicholas

AU - Pruitt, Rory

AU - Adams, Paul D.

AU - Chern, Mawsheng

AU - Petzold, Christopher J.

AU - Ronald , Pamela

PY - 2016

Y1 - 2016

N2 - Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.

AB - Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.

U2 - 10.1039/c5ib00232j

DO - 10.1039/c5ib00232j

M3 - Article

VL - 8

SP - 542

EP - 545

JO - Integrative Biology

JF - Integrative Biology

IS - 4

ER -

A second-generation expression system for tyrosine-sulfated proteins and its application in crop protection

Abstract

Access to Document

Fingerprint

Cite this