A specialised proteomic database for comparing matrix-assisted laser desorption/ionization-time of flight mass spectrometry data of tryptic peptides with corresponding sequence database segments

Georg F. Weiller*, Michael J. Djordjevic, Gilles Caraux, Hanchai Chen, Jeremy J. Weinman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

We have developed a specialised proteomic database for the analysis of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) data derived from tryptic peptides of Sinorhizobium meliloti proteins. This database currently contains the amino acid sequence data of the proteins predicted from the complete chromosome, MALDI-TOF MS data from proteolytic peptides of about 400 tryptically digested proteins, and the results of a search of the MALDI-TOF MS spectra against the chromosomal amino acid sequences. The database made it possible to access and compare the sequences of theoretical tryptic peptides that correspond to MALDI-TOF peaks in the mass spectrum with predicted tryptic peptides from identified proteins that could not be matched to MALDI-TOF peaks. A comparison of the molecular weights, isoelectric points and amino acid compositions of the identified and nonidentified peptides is presented. We also show how the system can assist in the development of an automated scoring function that facilitates and consolidates protein identification.

Original languageEnglish
Pages (from-to)1489-1494
Number of pages6
JournalProteomics
Volume1
Issue number12
DOIs
Publication statusPublished - Dec 2001

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