TY - JOUR
T1 - A study on the effect of cimetidine and l-carnitine on myoglobinuric acute kidney injury in male rats
AU - Estaphan, Suzanne
AU - Eissa, Hassan
AU - Elattar, Samah
AU - Rashed, Laila
AU - Farouk, Mira
N1 - Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Myoglobinuric acute renal failure is the most important life threatening complication of rhabdomyolysis. Iron, free radicals, nitric oxide and cytochrome p450 are involved in the pathogenesis of mARF. The aim of this study is to compare the effect of cimetidine, l-carnitine and both agents together on mARF in rats. Forty rats were divided into 5 groups; group I: control rats, group II: myoglobinuric ARF rats, group III: mARF rats received l-carnitine (200 mg/kg, i.p.), group IV: mARF rats received cimetidine (150 mg/kg i.p.) and group V: mARF rats received both agents together. 48 h after glycerol injection, systolic blood pressure was measured. Urine and blood samples were collected to evaluate urine volume, GFR, BUN, creatinine, K, Na, serum creatine kinase, NO and glutathione levels. Kidney specimens were taken to investigate renal cytochrome p450 and for histological examinations. Cimetidine treatment significantly decreased creatinine, BUN, K, Na, SBP and creatine kinase and increased GFR and urine volume compared to group II. l-carnitine exerted similar changes except for the effect on K and GFR. NO was significantly decreased, while renal glutathione and cytochrome p450 were significantly increased in groups treated with l-carnitine or cimetidine as compared to group II. Combined treatment further improved renal functions, creatine kinase, oxidative stress parameters and SBP as compared to each therapy alone. The histological changes confirmed the biochemical findings. Cimetidine and l-carnitine have protective effects - almost equally - against mARF. Using both agents together, minimises the renal injury.
AB - Myoglobinuric acute renal failure is the most important life threatening complication of rhabdomyolysis. Iron, free radicals, nitric oxide and cytochrome p450 are involved in the pathogenesis of mARF. The aim of this study is to compare the effect of cimetidine, l-carnitine and both agents together on mARF in rats. Forty rats were divided into 5 groups; group I: control rats, group II: myoglobinuric ARF rats, group III: mARF rats received l-carnitine (200 mg/kg, i.p.), group IV: mARF rats received cimetidine (150 mg/kg i.p.) and group V: mARF rats received both agents together. 48 h after glycerol injection, systolic blood pressure was measured. Urine and blood samples were collected to evaluate urine volume, GFR, BUN, creatinine, K, Na, serum creatine kinase, NO and glutathione levels. Kidney specimens were taken to investigate renal cytochrome p450 and for histological examinations. Cimetidine treatment significantly decreased creatinine, BUN, K, Na, SBP and creatine kinase and increased GFR and urine volume compared to group II. l-carnitine exerted similar changes except for the effect on K and GFR. NO was significantly decreased, while renal glutathione and cytochrome p450 were significantly increased in groups treated with l-carnitine or cimetidine as compared to group II. Combined treatment further improved renal functions, creatine kinase, oxidative stress parameters and SBP as compared to each therapy alone. The histological changes confirmed the biochemical findings. Cimetidine and l-carnitine have protective effects - almost equally - against mARF. Using both agents together, minimises the renal injury.
KW - Acute kidney injury
KW - Catalytic iron
KW - Cimetidine
KW - Cytochrome P450
KW - L-Carnitine
KW - Oxidative stress
KW - Rhabdomyolysis
UR - http://www.scopus.com/inward/record.url?scp=84933178497&partnerID=8YFLogxK
U2 - 10.1016/j.injury.2015.03.037
DO - 10.1016/j.injury.2015.03.037
M3 - Article
SN - 0020-1383
VL - 46
SP - 1223
EP - 1230
JO - Injury
JF - Injury
IS - 7
ER -