Absorbance Spectroscopy: Overview

Paul Wormell; Alison Rodger

doi:10.1007/978-3-642-16712-6_784

Absorbance Spectroscopy: Overview

Paul Wormell, Alison Rodger

Research output: Chapter in Book/Report/Conference proceeding › Entry for encyclopedia/dictionary › peer-review

1 Citation (Scopus)

Abstract

Synonyms
Absorbance of light; Transmission of light

Definition
Most spectroscopic phenomena arise from the interaction of a molecule with one photon at a time. The molecule may either absorb or scatter the photon; it may also emit a photon. The simplest form of spectroscopy is absorption, which measures how much light of a given frequency is absorbed by a collection of molecules. If a molecule absorbs a photon of frequency ν, it increases its energy by

ΔE = hν = hcλ (1)

where h is Planck’s constant, λ is the wavelength of the light, and c is the speed of light. Only light of the correct frequency to cause a molecule to jump from one energy level to another may be absorbed. When molecules absorb photons of visible or ultraviolet (UV) light, the magnitude of the energy of these photons is (2–12) × 10−19 J/molecule, λ= 170–800 nm which is the amount required to rearrange the valence electron distribution of a molecule. Thus, UV-visible absorption spectroscopy gives...

Original language	English
Title of host publication	Encyclopedia of Biophysics
Editors	Gordon C. K. Roberts
Place of Publication	Berlin, Heidelberg
Publisher	Springer Science+Business Media B.V.
Pages	23-25
Number of pages	3
ISBN (Electronic)	9783642167126
ISBN (Print)	9783642167119
DOIs	https://doi.org/10.1007/978-3-642-16712-6_784
Publication status	Published - 2013
Externally published	Yes

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10.1007/978-3-642-16712-6_784

Cite this

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abstract = "Synonyms Absorbance of light; Transmission of light Definition Most spectroscopic phenomena arise from the interaction of a molecule with one photon at a time. The molecule may either absorb or scatter the photon; it may also emit a photon. The simplest form of spectroscopy is absorption, which measures how much light of a given frequency is absorbed by a collection of molecules. If a molecule absorbs a photon of frequency ν, it increases its energy by ΔE = hν = hcλ (1) where h is Planck{\textquoteright}s constant, λ is the wavelength of the light, and c is the speed of light. Only light of the correct frequency to cause a molecule to jump from one energy level to another may be absorbed. When molecules absorb photons of visible or ultraviolet (UV) light, the magnitude of the energy of these photons is (2–12) × 10−19 J/molecule, λ= 170–800 nm which is the amount required to rearrange the valence electron distribution of a molecule. Thus, UV-visible absorption spectroscopy gives...",

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AB - Synonyms Absorbance of light; Transmission of light Definition Most spectroscopic phenomena arise from the interaction of a molecule with one photon at a time. The molecule may either absorb or scatter the photon; it may also emit a photon. The simplest form of spectroscopy is absorption, which measures how much light of a given frequency is absorbed by a collection of molecules. If a molecule absorbs a photon of frequency ν, it increases its energy by ΔE = hν = hcλ (1) where h is Planck’s constant, λ is the wavelength of the light, and c is the speed of light. Only light of the correct frequency to cause a molecule to jump from one energy level to another may be absorbed. When molecules absorb photons of visible or ultraviolet (UV) light, the magnitude of the energy of these photons is (2–12) × 10−19 J/molecule, λ= 170–800 nm which is the amount required to rearrange the valence electron distribution of a molecule. Thus, UV-visible absorption spectroscopy gives...

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ER -

Absorbance Spectroscopy: Overview

Abstract

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