@inproceedings{2115d9ae260349c39356bf398636f024,
title = "Achieving sub-micrometer imaging resolution in PDMS soft lithography devices using modified inverted selective plane illumination microscopy",
abstract = "We demonstrated light sheet fluorescence imaging at sub-micrometer resolution of biological samples enclosed in PDMS soft lithography devices. This was achieved by minimizing the optical distortion induced at the external PDMS device interface using a sucrose solution with refractive index (RI) matched to the PDMS. A commercially available inverted Selective Plane Illumination Microscopy (iSPIM) was modified with a single mode fiber (SMF) and an electrically tunable lens (ETL) module to facilitate RI matching and compensate for sucrose solution induced axial focal shift. We termed this protocol as the modified iSPIM (m-iSPIM).",
keywords = "ISPIM, Light sheet imaging, PDMS device, QPM",
author = "Tienan Xu and Lim, {Yean Jin} and Yujie Zheng and Jung, {Moon Sun} and Katharina Gaus and Gardiner, {Elizabeth E.} and Lee, {Woei Ming}",
note = "Publisher Copyright: {\textcopyright} 2020 CBMS-0001; 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",
year = "2020",
language = "English",
series = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "558--560",
booktitle = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}