Alternative splicing within the I-II loop controls surface expression of T-type Ca(v)3.1 calcium channels

Aleksandr Shcheglovitov; Iuliia Vitko; Isabelle Bidaud; Joel P Baumgart; Manuel Navarro-Gonzalez; Thomas Grayson; Philippe Lory; Caryl Hill; Edward Perez-Reyes

doi:10.1016/j.febslet.2008.10.013

Alternative splicing within the I-II loop controls surface expression of T-type Ca(v)3.1 calcium channels

Aleksandr Shcheglovitov, Iuliia Vitko, Isabelle Bidaud, Joel P Baumgart, Manuel Navarro-Gonzalez, Thomas Grayson, Philippe Lory, Caryl Hill, Edward Perez-Reyes

Research output: Contribution to journal › Article › peer-review

Abstract

Molecular diversity of T-type/Cav3 Ca2+ channels is created by expression of three genes and alternative splicing of those genes. Prompted by the important role of the I-II linker in gating and surface expression of Cav3 channels, we describe here the properties of a novel variant that partially deletes this loop. The variant is abundantly expressed in rat brain, even exceeding transcripts with the complete exon 8. Electrophysiological analysis of the Î”8b variant revealed enhanced current density compared to Cav3.1a, but similar gating. Luminometry experiments revealed an increase in the expression of Î”8b channels at the plasma membrane. We conclude that alternative splicing of Cav3 channels regulates surface expression and may underlie disease states in which T-channel current density is increased.

Original language	English
Pages (from-to)	2765-3770
Journal	FEBS Letters
Volume	582
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2008.10.013
Publication status	Published - 2008

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title = "Alternative splicing within the I-II loop controls surface expression of T-type Ca(v)3.1 calcium channels",

abstract = "Molecular diversity of T-type/Cav3 Ca2+ channels is created by expression of three genes and alternative splicing of those genes. Prompted by the important role of the I-II linker in gating and surface expression of Cav3 channels, we describe here the properties of a novel variant that partially deletes this loop. The variant is abundantly expressed in rat brain, even exceeding transcripts with the complete exon 8. Electrophysiological analysis of the {\^I}”8b variant revealed enhanced current density compared to Cav3.1a, but similar gating. Luminometry experiments revealed an increase in the expression of {\^I}”8b channels at the plasma membrane. We conclude that alternative splicing of Cav3 channels regulates surface expression and may underlie disease states in which T-channel current density is increased.",

author = "Aleksandr Shcheglovitov and Iuliia Vitko and Isabelle Bidaud and Baumgart, {Joel P} and Manuel Navarro-Gonzalez and Thomas Grayson and Philippe Lory and Caryl Hill and Edward Perez-Reyes",

year = "2008",

doi = "10.1016/j.febslet.2008.10.013",

language = "English",

volume = "582",

pages = "2765--3770",

journal = "FEBS Letters",

publisher = "Elsevier",

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}

TY - JOUR

T1 - Alternative splicing within the I-II loop controls surface expression of T-type Ca(v)3.1 calcium channels

AU - Shcheglovitov, Aleksandr

AU - Vitko, Iuliia

AU - Bidaud, Isabelle

AU - Baumgart, Joel P

AU - Navarro-Gonzalez, Manuel

AU - Grayson, Thomas

AU - Lory, Philippe

AU - Hill, Caryl

AU - Perez-Reyes, Edward

PY - 2008

Y1 - 2008

N2 - Molecular diversity of T-type/Cav3 Ca2+ channels is created by expression of three genes and alternative splicing of those genes. Prompted by the important role of the I-II linker in gating and surface expression of Cav3 channels, we describe here the properties of a novel variant that partially deletes this loop. The variant is abundantly expressed in rat brain, even exceeding transcripts with the complete exon 8. Electrophysiological analysis of the Î”8b variant revealed enhanced current density compared to Cav3.1a, but similar gating. Luminometry experiments revealed an increase in the expression of Î”8b channels at the plasma membrane. We conclude that alternative splicing of Cav3 channels regulates surface expression and may underlie disease states in which T-channel current density is increased.

AB - Molecular diversity of T-type/Cav3 Ca2+ channels is created by expression of three genes and alternative splicing of those genes. Prompted by the important role of the I-II linker in gating and surface expression of Cav3 channels, we describe here the properties of a novel variant that partially deletes this loop. The variant is abundantly expressed in rat brain, even exceeding transcripts with the complete exon 8. Electrophysiological analysis of the Î”8b variant revealed enhanced current density compared to Cav3.1a, but similar gating. Luminometry experiments revealed an increase in the expression of Î”8b channels at the plasma membrane. We conclude that alternative splicing of Cav3 channels regulates surface expression and may underlie disease states in which T-channel current density is increased.

U2 - 10.1016/j.febslet.2008.10.013

DO - 10.1016/j.febslet.2008.10.013

M3 - Article

VL - 582

SP - 2765

EP - 3770

JO - FEBS Letters

JF - FEBS Letters

IS - 27

ER -

Alternative splicing within the I-II loop controls surface expression of T-type Ca(v)3.1 calcium channels

Abstract

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