An alternative oxidase monoclonal antibody recognises a highly conserved sequence among alternative oxidase subunits

Patrick M. Finnegan; Anthea R. Wooding; David A. Day

doi:10.1016/S0014-5793(99)00259-8

An alternative oxidase monoclonal antibody recognises a highly conserved sequence among alternative oxidase subunits

Patrick M. Finnegan^*, Anthea R. Wooding, David A. Day

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The alternative oxidase is found in the inner mitochondrial membranes of plants and some fungi and protists. A monoclonal antibody raised against the alternative oxidase from the aroid lily Sauromatum guttatum has been used extensively to detect the enzyme in these organisms. Using an immunoblotting strategy, the antibody binding site has been localised to the sequence RADEAHHRDVNH within the soybean alternative oxidase 2 protein. Examination of sequence variants showed that A2 and residues C-terminal to H7 are required for recognition by the monoclonal antibody raised against the alternative oxidase. The recognition sequence is highly conserved among all alternative oxidase proteins and is absolutely conserved in 12 of 14 higher plant sequences, suggesting that this antibody will continue to be extremely useful in studying the expression and synthesis of the alternative oxidase. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	21-24
Number of pages	4
Journal	FEBS Letters
Volume	447
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(99)00259-8
Publication status	Published - 19 Mar 1999

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title = "An alternative oxidase monoclonal antibody recognises a highly conserved sequence among alternative oxidase subunits",

abstract = "The alternative oxidase is found in the inner mitochondrial membranes of plants and some fungi and protists. A monoclonal antibody raised against the alternative oxidase from the aroid lily Sauromatum guttatum has been used extensively to detect the enzyme in these organisms. Using an immunoblotting strategy, the antibody binding site has been localised to the sequence RADEAHHRDVNH within the soybean alternative oxidase 2 protein. Examination of sequence variants showed that A2 and residues C-terminal to H7 are required for recognition by the monoclonal antibody raised against the alternative oxidase. The recognition sequence is highly conserved among all alternative oxidase proteins and is absolutely conserved in 12 of 14 higher plant sequences, suggesting that this antibody will continue to be extremely useful in studying the expression and synthesis of the alternative oxidase. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Alternative oxidase, Epitope, Higher plant, Mitochondrium, Protozoan parasite, Respiration",

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T1 - An alternative oxidase monoclonal antibody recognises a highly conserved sequence among alternative oxidase subunits

AU - Finnegan, Patrick M.

AU - Wooding, Anthea R.

AU - Day, David A.

PY - 1999/3/19

Y1 - 1999/3/19

N2 - The alternative oxidase is found in the inner mitochondrial membranes of plants and some fungi and protists. A monoclonal antibody raised against the alternative oxidase from the aroid lily Sauromatum guttatum has been used extensively to detect the enzyme in these organisms. Using an immunoblotting strategy, the antibody binding site has been localised to the sequence RADEAHHRDVNH within the soybean alternative oxidase 2 protein. Examination of sequence variants showed that A2 and residues C-terminal to H7 are required for recognition by the monoclonal antibody raised against the alternative oxidase. The recognition sequence is highly conserved among all alternative oxidase proteins and is absolutely conserved in 12 of 14 higher plant sequences, suggesting that this antibody will continue to be extremely useful in studying the expression and synthesis of the alternative oxidase. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - The alternative oxidase is found in the inner mitochondrial membranes of plants and some fungi and protists. A monoclonal antibody raised against the alternative oxidase from the aroid lily Sauromatum guttatum has been used extensively to detect the enzyme in these organisms. Using an immunoblotting strategy, the antibody binding site has been localised to the sequence RADEAHHRDVNH within the soybean alternative oxidase 2 protein. Examination of sequence variants showed that A2 and residues C-terminal to H7 are required for recognition by the monoclonal antibody raised against the alternative oxidase. The recognition sequence is highly conserved among all alternative oxidase proteins and is absolutely conserved in 12 of 14 higher plant sequences, suggesting that this antibody will continue to be extremely useful in studying the expression and synthesis of the alternative oxidase. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Alternative oxidase

KW - Epitope

KW - Higher plant

KW - Mitochondrium

KW - Protozoan parasite

KW - Respiration

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DO - 10.1016/S0014-5793(99)00259-8

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JO - FEBS Letters

JF - FEBS Letters

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An alternative oxidase monoclonal antibody recognises a highly conserved sequence among alternative oxidase subunits

Abstract

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