An autoactive NB-LRR gene causes Rht13 dwarfism in wheat

Philippa Borrill; Rohit Mago; Tianyuan Xu; Brett Ford; Simon J. Williams; Adinda Derkx; William D. Bovill; Jessica Hyles; Dhara Bhatt; Xiaodi Xia; Colleen MacMillan; Rosemary White; Wolfram Buss; István Molnár; Sean Walkowiak; Odd Arne Olsen; Jaroslav Doležel; Curtis J. Pozniak; Wolfgang Spielmeyer

doi:10.1073/pnas.2209875119

An autoactive NB-LRR gene causes Rht13 dwarfism in wheat

Philippa Borrill^*, Rohit Mago, Tianyuan Xu, Brett Ford, Simon J. Williams, Adinda Derkx, William D. Bovill, Jessica Hyles, Dhara Bhatt, Xiaodi Xia, Colleen MacMillan, Rosemary White, Wolfram Buss, István Molnár, Sean Walkowiak, Odd Arne Olsen, Jaroslav Doležel, Curtis J. Pozniak, Wolfgang Spielmeyer

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat (NB-LRR) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height (Rht) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker.

Original language	English
Article number	e2209875119
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	119
Issue number	48
DOIs	https://doi.org/10.1073/pnas.2209875119
Publication status	Published - 29 Nov 2022

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Borrill, P., Mago, R., Xu, T., Ford, B., Williams, S. J., Derkx, A., Bovill, W. D., Hyles, J., Bhatt, D., Xia, X., MacMillan, C., White, R., Buss, W., Molnár, I., Walkowiak, S., Olsen, O. A., Doležel, J., Pozniak, C. J., & Spielmeyer, W. (2022). An autoactive NB-LRR gene causes Rht13 dwarfism in wheat. Proceedings of the National Academy of Sciences of the United States of America, 119(48), Article e2209875119. https://doi.org/10.1073/pnas.2209875119

@article{264a288c62954f95bab41c4220253d54,

title = "An autoactive NB-LRR gene causes Rht13 dwarfism in wheat",

abstract = "Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat (NB-LRR) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height (Rht) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker.",

keywords = "Triticum aestivum L. (wheat), autoactive NB-LRR, reduced-height (Rht) gene, semidwarfing gene",

author = "Philippa Borrill and Rohit Mago and Tianyuan Xu and Brett Ford and Williams, {Simon J.} and Adinda Derkx and Bovill, {William D.} and Jessica Hyles and Dhara Bhatt and Xiaodi Xia and Colleen MacMillan and Rosemary White and Wolfram Buss and Istv{\'a}n Moln{\'a}r and Sean Walkowiak and Olsen, {Odd Arne} and Jaroslav Dole{\v z}el and Pozniak, {Curtis J.} and Wolfgang Spielmeyer",

note = "Publisher Copyright: Copyright {\textcopyright} 2022 the Author(s). Published by PNAS.",

year = "2022",

month = nov,

day = "29",

doi = "10.1073/pnas.2209875119",

language = "English",

volume = "119",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

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Borrill, P, Mago, R, Xu, T, Ford, B, Williams, SJ, Derkx, A, Bovill, WD, Hyles, J, Bhatt, D, Xia, X, MacMillan, C, White, R, Buss, W, Molnár, I, Walkowiak, S, Olsen, OA, Doležel, J, Pozniak, CJ & Spielmeyer, W 2022, 'An autoactive NB-LRR gene causes Rht13 dwarfism in wheat', Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 48, e2209875119. https://doi.org/10.1073/pnas.2209875119

TY - JOUR

T1 - An autoactive NB-LRR gene causes Rht13 dwarfism in wheat

AU - Borrill, Philippa

AU - Mago, Rohit

AU - Xu, Tianyuan

AU - Ford, Brett

AU - Williams, Simon J.

AU - Derkx, Adinda

AU - Bovill, William D.

AU - Hyles, Jessica

AU - Bhatt, Dhara

AU - Xia, Xiaodi

AU - MacMillan, Colleen

AU - White, Rosemary

AU - Buss, Wolfram

AU - Molnár, István

AU - Walkowiak, Sean

AU - Olsen, Odd Arne

AU - Doležel, Jaroslav

AU - Pozniak, Curtis J.

AU - Spielmeyer, Wolfgang

PY - 2022/11/29

Y1 - 2022/11/29

N2 - Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat (NB-LRR) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height (Rht) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker.

AB - Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat (NB-LRR) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height (Rht) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker.

KW - Triticum aestivum L. (wheat)

KW - autoactive NB-LRR

KW - reduced-height (Rht) gene

KW - semidwarfing gene

UR - http://www.scopus.com/inward/record.url?scp=85142939678&partnerID=8YFLogxK

U2 - 10.1073/pnas.2209875119

DO - 10.1073/pnas.2209875119

M3 - Article

SN - 0027-8424

VL - 119

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 48

M1 - e2209875119

ER -

An autoactive NB-LRR gene causes Rht13 dwarfism in wheat

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