An economical adaptation of the RosetteSep™ procedure for NK cell enrichment from whole blood, and its use with liquid nitrogen stored peripheral blood mononuclear cells

Hilary S. Warren; Purna M. Rana

doi:10.1016/S0022-1759(03)00106-6

An economical adaptation of the RosetteSep™ procedure for NK cell enrichment from whole blood, and its use with liquid nitrogen stored peripheral blood mononuclear cells

Hilary S. Warren^*, Purna M. Rana

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

This study reports an economical adaptation of the RosetteSep™ procedure for enrichment of NK cells designed for whole blood and its use with liquid nitrogen stored peripheral blood mononuclear cells (PBMC). Combining 45×10⁶ PBMC with 45×10⁸ Alsevers' stored red blood cells (RBC) in 1 ml requires 50 μl of RosetteSep™ bifunctional antibody cocktail and provides NK cells of 99% purity with average yields of 43%.

Original language	English
Pages (from-to)	135-138
Number of pages	4
Journal	Journal of Immunological Methods
Volume	280
Issue number	1-2
DOIs	https://doi.org/10.1016/S0022-1759(03)00106-6
Publication status	Published - 1 Sept 2003

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title = "An economical adaptation of the RosetteSep{\texttrademark} procedure for NK cell enrichment from whole blood, and its use with liquid nitrogen stored peripheral blood mononuclear cells",

abstract = "This study reports an economical adaptation of the RosetteSep{\texttrademark} procedure for enrichment of NK cells designed for whole blood and its use with liquid nitrogen stored peripheral blood mononuclear cells (PBMC). Combining 45×106 PBMC with 45×108 Alsevers' stored red blood cells (RBC) in 1 ml requires 50 μl of RosetteSep{\texttrademark} bifunctional antibody cocktail and provides NK cells of 99\% purity with average yields of 43\%.",

keywords = "Lymphocyte isolation, NK cells, Rosette separation",

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AU - Warren, Hilary S.

AU - Rana, Purna M.

PY - 2003/9/1

Y1 - 2003/9/1

N2 - This study reports an economical adaptation of the RosetteSep™ procedure for enrichment of NK cells designed for whole blood and its use with liquid nitrogen stored peripheral blood mononuclear cells (PBMC). Combining 45×106 PBMC with 45×108 Alsevers' stored red blood cells (RBC) in 1 ml requires 50 μl of RosetteSep™ bifunctional antibody cocktail and provides NK cells of 99% purity with average yields of 43%.

AB - This study reports an economical adaptation of the RosetteSep™ procedure for enrichment of NK cells designed for whole blood and its use with liquid nitrogen stored peripheral blood mononuclear cells (PBMC). Combining 45×106 PBMC with 45×108 Alsevers' stored red blood cells (RBC) in 1 ml requires 50 μl of RosetteSep™ bifunctional antibody cocktail and provides NK cells of 99% purity with average yields of 43%.

KW - Lymphocyte isolation

KW - NK cells

KW - Rosette separation

UR - https://www.scopus.com/pages/publications/0041695940

U2 - 10.1016/S0022-1759(03)00106-6

DO - 10.1016/S0022-1759(03)00106-6

M3 - Article

SN - 0022-1759

VL - 280

SP - 135

EP - 138

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

An economical adaptation of the RosetteSep™ procedure for NK cell enrichment from whole blood, and its use with liquid nitrogen stored peripheral blood mononuclear cells

Abstract

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