An environmental DNA assay for detecting Macquarie perch, Macquaria australasica

Maxine P. Piggott

doi:10.1007/s12686-016-0666-0

An environmental DNA assay for detecting Macquarie perch, Macquaria australasica

Maxine P. Piggott^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Macquarie perch (Macquaria australasica) are an endangered freshwater fish found in the Murray-Darling Basin, Australia. I developed a species-specific assay to detect Macquarie perch DNA in environmental samples. The assay is highly specific to detect Macquarie perch, and produced no amplification from 18 co-distributed species. Testing the assay on environmental water samples demonstrated positive detections where Macquarie perch are known to be present in low and moderate density.

Original language	English
Pages (from-to)	257-259
Number of pages	3
Journal	Conservation Genetics Resources
Volume	9
Issue number	2
DOIs	https://doi.org/10.1007/s12686-016-0666-0
Publication status	Published - 1 Jun 2017

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title = "An environmental DNA assay for detecting Macquarie perch, Macquaria australasica",

abstract = "Macquarie perch (Macquaria australasica) are an endangered freshwater fish found in the Murray-Darling Basin, Australia. I developed a species-specific assay to detect Macquarie perch DNA in environmental samples. The assay is highly specific to detect Macquarie perch, and produced no amplification from 18 co-distributed species. Testing the assay on environmental water samples demonstrated positive detections where Macquarie perch are known to be present in low and moderate density.",

keywords = "Environmental DNA, Macquaria australasica, Macquarie perch, eDNA, qPCR",

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AB - Macquarie perch (Macquaria australasica) are an endangered freshwater fish found in the Murray-Darling Basin, Australia. I developed a species-specific assay to detect Macquarie perch DNA in environmental samples. The assay is highly specific to detect Macquarie perch, and produced no amplification from 18 co-distributed species. Testing the assay on environmental water samples demonstrated positive detections where Macquarie perch are known to be present in low and moderate density.

KW - Environmental DNA

KW - Macquaria australasica

KW - Macquarie perch

KW - eDNA

KW - qPCR

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ER -

An environmental DNA assay for detecting Macquarie perch, Macquaria australasica

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