TY - JOUR
T1 - An expanded database of high-resolution MS/MS spectra for lichen-derived natural products
AU - Bracegirdle, Joe
AU - Elix, John A.
AU - Mawalagedera, Udayangani
AU - Chooi, Yit Heng
AU - Gueidan, Cécile
N1 - © The Author(s) 2025.
PY - 2025/2/12
Y1 - 2025/2/12
N2 - The history of lichen compound identification has long relied on techniques such as spot tests and TLC, which have been surpassed in sensitivity and accuracy by modern metabolomic techniques such as high-resolution MS/MS. In 2019, Olivier-Jimenez et al. released the Lichen DataBase (LDB), a library containing the Q-TOF MS/MS spectra of 251 metabolites on the MetaboLights and GNPS platforms, that has been widely used for the identification of lichen-derived unknowns. To increase the compound coverage, we have generated the Orbitrap MS/MS spectra of a further 534 lichen-derived compounds from the metabolite library of Jack Elix, housed at the CANB herbarium (Canberra, Australia). This included 399 unique metabolites that are not in the LDB, bringing the total number combined to 650. Technical validation was achieved by investigating the compounds in three Australian lichen extracts using the Library Search and Molecular Networking tools on the GNPS platform. This update provides a much larger database for lichen compound identification, which we envisage will allow refining the lichen chemotaxonomy framework and contribute to compound discovery.
AB - The history of lichen compound identification has long relied on techniques such as spot tests and TLC, which have been surpassed in sensitivity and accuracy by modern metabolomic techniques such as high-resolution MS/MS. In 2019, Olivier-Jimenez et al. released the Lichen DataBase (LDB), a library containing the Q-TOF MS/MS spectra of 251 metabolites on the MetaboLights and GNPS platforms, that has been widely used for the identification of lichen-derived unknowns. To increase the compound coverage, we have generated the Orbitrap MS/MS spectra of a further 534 lichen-derived compounds from the metabolite library of Jack Elix, housed at the CANB herbarium (Canberra, Australia). This included 399 unique metabolites that are not in the LDB, bringing the total number combined to 650. Technical validation was achieved by investigating the compounds in three Australian lichen extracts using the Library Search and Molecular Networking tools on the GNPS platform. This update provides a much larger database for lichen compound identification, which we envisage will allow refining the lichen chemotaxonomy framework and contribute to compound discovery.
UR - http://www.scopus.com/inward/record.url?scp=85218838706&partnerID=8YFLogxK
U2 - 10.1038/s41597-025-04488-w
DO - 10.1038/s41597-025-04488-w
M3 - Article
C2 - 39934125
AN - SCOPUS:85218838706
SN - 2052-4463
VL - 12
JO - Scientific data
JF - Scientific data
IS - 1
M1 - 244
ER -