An XA21-associated kinase (OsSERK2) regulates immunity mediated by the XA21 and XA3 immune receptors

Xuewei Chen; Shimin Zuo; Benjamin Schwessinger; Mawsheng Chern; Patrick E. Canlas; Deling Ruan; Xiaogang Zhou; Jingrfpy Wang; Arsalan Daudi; Christopher J. Petzold; Joshua L. Heazlewood; Pamela C. Ronald

doi:10.1093/mp/ssu003

An XA21-associated kinase (OsSERK2) regulates immunity mediated by the XA21 and XA3 immune receptors

Xuewei Chen^*, Shimin Zuo, Benjamin Schwessinger, Mawsheng Chern, Patrick E. Canlas, Deling Ruan, Xiaogang Zhou, Jingrfpy Wang, Arsalan Daudi, Christopher J. Petzold, Joshua L. Heazlewood, Pamela C. Ronald

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

132 Citations (Scopus)

Abstract

The rice XA21 immune receptor kinase and the structurally related XA3 receptor confer immunity to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial leaf blight. Here we report the isolation of OsSERK2 (rice somatic embryogenesis receptor kinase 2) and demonstrate that OsSERK2 positively regulates immunity mediated by XA21 and XA3 as well as the rice immune receptor FLS2 (OsFLS2). Rice plants silenced for OsSerk2 display altered morphology and reduced sensitivity to the hormone brassinolide. OsSERK2 interacts with the intracellular domains of each immune receptor in the yeast two-hybrid system in a kinase activity-dependent manner. OsSERK2 undergoes bidirectional transphosphorylation with XA21 in vitro and forms a constitutive complex with XA21 in vivo. These results demonstrate an essential role for OsSERK2 in the function of three rice immune receptors and suggest that direct interaction with the rice immune receptors is critical for their function. Taken together, our findings suggest that the mechanism of OsSERK2-meditated regulation of rice XA21, XA3, and FLS2 differs from that of AtSERK3/BAK1-mediated regulation of Arabidopsis FLS2 and EFR.

Original language	English
Pages (from-to)	874-892
Number of pages	19
Journal	Molecular Plant
Volume	7
Issue number	5
DOIs	https://doi.org/10.1093/mp/ssu003
Publication status	Published - May 2014
Externally published	Yes

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@article{a469462d0483416b8909a0ad41b4d964,

title = "An XA21-associated kinase (OsSERK2) regulates immunity mediated by the XA21 and XA3 immune receptors",

abstract = "The rice XA21 immune receptor kinase and the structurally related XA3 receptor confer immunity to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial leaf blight. Here we report the isolation of OsSERK2 (rice somatic embryogenesis receptor kinase 2) and demonstrate that OsSERK2 positively regulates immunity mediated by XA21 and XA3 as well as the rice immune receptor FLS2 (OsFLS2). Rice plants silenced for OsSerk2 display altered morphology and reduced sensitivity to the hormone brassinolide. OsSERK2 interacts with the intracellular domains of each immune receptor in the yeast two-hybrid system in a kinase activity-dependent manner. OsSERK2 undergoes bidirectional transphosphorylation with XA21 in vitro and forms a constitutive complex with XA21 in vivo. These results demonstrate an essential role for OsSERK2 in the function of three rice immune receptors and suggest that direct interaction with the rice immune receptors is critical for their function. Taken together, our findings suggest that the mechanism of OsSERK2-meditated regulation of rice XA21, XA3, and FLS2 differs from that of AtSERK3/BAK1-mediated regulation of Arabidopsis FLS2 and EFR.",

keywords = "Xanthomonas oryzae pv. oryzae, immune receptor kinases, immunity, rice, somatic embryogenesis receptor kinase (SERK)",

author = "Xuewei Chen and Shimin Zuo and Benjamin Schwessinger and Mawsheng Chern and Canlas, {Patrick E.} and Deling Ruan and Xiaogang Zhou and Jingrfpy Wang and Arsalan Daudi and Petzold, {Christopher J.} and Heazlewood, {Joshua L.} and Ronald, {Pamela C.}",

year = "2014",

month = may,

doi = "10.1093/mp/ssu003",

language = "English",

volume = "7",

pages = "874--892",

journal = "Molecular Plant",

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number = "5",

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T1 - An XA21-associated kinase (OsSERK2) regulates immunity mediated by the XA21 and XA3 immune receptors

AU - Chen, Xuewei

AU - Zuo, Shimin

AU - Schwessinger, Benjamin

AU - Chern, Mawsheng

AU - Canlas, Patrick E.

AU - Ruan, Deling

AU - Zhou, Xiaogang

AU - Wang, Jingrfpy

AU - Daudi, Arsalan

AU - Petzold, Christopher J.

AU - Heazlewood, Joshua L.

AU - Ronald, Pamela C.

PY - 2014/5

Y1 - 2014/5

N2 - The rice XA21 immune receptor kinase and the structurally related XA3 receptor confer immunity to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial leaf blight. Here we report the isolation of OsSERK2 (rice somatic embryogenesis receptor kinase 2) and demonstrate that OsSERK2 positively regulates immunity mediated by XA21 and XA3 as well as the rice immune receptor FLS2 (OsFLS2). Rice plants silenced for OsSerk2 display altered morphology and reduced sensitivity to the hormone brassinolide. OsSERK2 interacts with the intracellular domains of each immune receptor in the yeast two-hybrid system in a kinase activity-dependent manner. OsSERK2 undergoes bidirectional transphosphorylation with XA21 in vitro and forms a constitutive complex with XA21 in vivo. These results demonstrate an essential role for OsSERK2 in the function of three rice immune receptors and suggest that direct interaction with the rice immune receptors is critical for their function. Taken together, our findings suggest that the mechanism of OsSERK2-meditated regulation of rice XA21, XA3, and FLS2 differs from that of AtSERK3/BAK1-mediated regulation of Arabidopsis FLS2 and EFR.

AB - The rice XA21 immune receptor kinase and the structurally related XA3 receptor confer immunity to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial leaf blight. Here we report the isolation of OsSERK2 (rice somatic embryogenesis receptor kinase 2) and demonstrate that OsSERK2 positively regulates immunity mediated by XA21 and XA3 as well as the rice immune receptor FLS2 (OsFLS2). Rice plants silenced for OsSerk2 display altered morphology and reduced sensitivity to the hormone brassinolide. OsSERK2 interacts with the intracellular domains of each immune receptor in the yeast two-hybrid system in a kinase activity-dependent manner. OsSERK2 undergoes bidirectional transphosphorylation with XA21 in vitro and forms a constitutive complex with XA21 in vivo. These results demonstrate an essential role for OsSERK2 in the function of three rice immune receptors and suggest that direct interaction with the rice immune receptors is critical for their function. Taken together, our findings suggest that the mechanism of OsSERK2-meditated regulation of rice XA21, XA3, and FLS2 differs from that of AtSERK3/BAK1-mediated regulation of Arabidopsis FLS2 and EFR.

KW - Xanthomonas oryzae pv. oryzae

KW - immune receptor kinases

KW - immunity

KW - rice

KW - somatic embryogenesis receptor kinase (SERK)

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U2 - 10.1093/mp/ssu003

DO - 10.1093/mp/ssu003

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SN - 1674-2052

VL - 7

SP - 874

EP - 892

JO - Molecular Plant

JF - Molecular Plant

IS - 5

ER -

An XA21-associated kinase (OsSERK2) regulates immunity mediated by the XA21 and XA3 immune receptors

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