Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging

Anthony C. Jones; Bruce Milthorpe; Holger Averdunk; Ajay Limaye; Tim J. Senden; Arthur Sakellariou; Adrian P. Sheppard; Rob M. Sok; Mark A. Knackstedt; Arthur Brandwood; Dennis Rohner; Dietmar W. Hutmacher

doi:10.1016/j.biomaterials.2004.01.047

Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging

Anthony C. Jones, Bruce Milthorpe^*, Holger Averdunk, Ajay Limaye, Tim J. Senden, Arthur Sakellariou, Adrian P. Sheppard, Rob M. Sok, Mark A. Knackstedt, Arthur Brandwood, Dennis Rohner, Dietmar W. Hutmacher

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

159 Citations (Scopus)

Abstract

This paper illustrates the utility of micro-computed tomography (micro-CT) to study the process of tissue engineered bone growth. A micro-CT facility for imaging and visualising biomaterials in three dimensions (3D) is described. The facility is capable of acquiring 3D images made up of 2000³ voxels on specimens up to 60mm in extent with resolutions down to 2μm. This allows the 3D structure of tissue engineered materials to be imaged across three orders of magnitude of detail. The capabilities of micro-CT are demonstrated by imaging the Haversian network within human femoral cortical bone (distal diaphysis) and bone ingrowth into a porous scaffold at varying resolutions. Phase identification combined with 3D visualisation enables one to observe the complex topology of the canalicular system of the cortical bone. Imaging of the tissue engineered bone at a scale of 1cm and resolutions of 10μm allows visualisation of the complex ingrowth of bone into the polymer scaffold. Further imaging at 2μm resolution allows observation of bone ultra-structure. These observations illustrate the benefits of tomography over traditional techniques for the characterisation of bone morphology and interconnectivity and performs a complimentary role to current histomorphometric techniques.

Original language	English
Pages (from-to)	4947-4954
Number of pages	8
Journal	Biomaterials
Volume	25
Issue number	20
DOIs	https://doi.org/10.1016/j.biomaterials.2004.01.047
Publication status	Published - Sept 2004

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10.1016/j.biomaterials.2004.01.047

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title = "Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging",

abstract = "This paper illustrates the utility of micro-computed tomography (micro-CT) to study the process of tissue engineered bone growth. A micro-CT facility for imaging and visualising biomaterials in three dimensions (3D) is described. The facility is capable of acquiring 3D images made up of 20003 voxels on specimens up to 60mm in extent with resolutions down to 2μm. This allows the 3D structure of tissue engineered materials to be imaged across three orders of magnitude of detail. The capabilities of micro-CT are demonstrated by imaging the Haversian network within human femoral cortical bone (distal diaphysis) and bone ingrowth into a porous scaffold at varying resolutions. Phase identification combined with 3D visualisation enables one to observe the complex topology of the canalicular system of the cortical bone. Imaging of the tissue engineered bone at a scale of 1cm and resolutions of 10μm allows visualisation of the complex ingrowth of bone into the polymer scaffold. Further imaging at 2μm resolution allows observation of bone ultra-structure. These observations illustrate the benefits of tomography over traditional techniques for the characterisation of bone morphology and interconnectivity and performs a complimentary role to current histomorphometric techniques.",

keywords = "Bone tissue engineering, Image analysis, Micro-structure, Scaffold, X-ray micro-computed tomography",

author = "Jones, {Anthony C.} and Bruce Milthorpe and Holger Averdunk and Ajay Limaye and Senden, {Tim J.} and Arthur Sakellariou and Sheppard, {Adrian P.} and Sok, {Rob M.} and Knackstedt, {Mark A.} and Arthur Brandwood and Dennis Rohner and Hutmacher, {Dietmar W.}",

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AU - Averdunk, Holger

AU - Limaye, Ajay

AU - Senden, Tim J.

AU - Sakellariou, Arthur

AU - Sheppard, Adrian P.

AU - Sok, Rob M.

AU - Knackstedt, Mark A.

AU - Brandwood, Arthur

AU - Rohner, Dennis

AU - Hutmacher, Dietmar W.

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N2 - This paper illustrates the utility of micro-computed tomography (micro-CT) to study the process of tissue engineered bone growth. A micro-CT facility for imaging and visualising biomaterials in three dimensions (3D) is described. The facility is capable of acquiring 3D images made up of 20003 voxels on specimens up to 60mm in extent with resolutions down to 2μm. This allows the 3D structure of tissue engineered materials to be imaged across three orders of magnitude of detail. The capabilities of micro-CT are demonstrated by imaging the Haversian network within human femoral cortical bone (distal diaphysis) and bone ingrowth into a porous scaffold at varying resolutions. Phase identification combined with 3D visualisation enables one to observe the complex topology of the canalicular system of the cortical bone. Imaging of the tissue engineered bone at a scale of 1cm and resolutions of 10μm allows visualisation of the complex ingrowth of bone into the polymer scaffold. Further imaging at 2μm resolution allows observation of bone ultra-structure. These observations illustrate the benefits of tomography over traditional techniques for the characterisation of bone morphology and interconnectivity and performs a complimentary role to current histomorphometric techniques.

AB - This paper illustrates the utility of micro-computed tomography (micro-CT) to study the process of tissue engineered bone growth. A micro-CT facility for imaging and visualising biomaterials in three dimensions (3D) is described. The facility is capable of acquiring 3D images made up of 20003 voxels on specimens up to 60mm in extent with resolutions down to 2μm. This allows the 3D structure of tissue engineered materials to be imaged across three orders of magnitude of detail. The capabilities of micro-CT are demonstrated by imaging the Haversian network within human femoral cortical bone (distal diaphysis) and bone ingrowth into a porous scaffold at varying resolutions. Phase identification combined with 3D visualisation enables one to observe the complex topology of the canalicular system of the cortical bone. Imaging of the tissue engineered bone at a scale of 1cm and resolutions of 10μm allows visualisation of the complex ingrowth of bone into the polymer scaffold. Further imaging at 2μm resolution allows observation of bone ultra-structure. These observations illustrate the benefits of tomography over traditional techniques for the characterisation of bone morphology and interconnectivity and performs a complimentary role to current histomorphometric techniques.

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KW - Micro-structure

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KW - X-ray micro-computed tomography

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JO - Biomaterials

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Analysis of 3D bone ingrowth into polymer scaffolds via micro-computed tomography imaging

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