Analyzing CD8 T cells in mouse models of poxvirus infection

Inge E.A. Flesch, Yik Chun Wong, David C. Tscharke

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    9 Citations (Scopus)


    Mouse models of immunology are frequently used to study host responses to poxviruses or poxvirus-based recombinant vaccines. In this context, the magnitude of CD8+ T cell responses is often of interest. Methods to evaluate CD8+ T cell responses extend from those that rely on indirect measurement of effector function only, such as cytotoxicity assays, to those that only measure antiviral CD8+ T cell numbers and not function, like peptide MHC tetramers. In this chapter, five methods are provided that cover this range: DimerX staining (a variant of peptide-MHC tetramers), intracellular cytokine staining for interferon-γ, CD62L/Granzyme B staining, and in vitro and ex vivo cytotoxicity assays. We also include tables of vaccinia virus peptide epitopes for use in most of these assays.

    Original languageEnglish
    Title of host publicationVaccinia Virus and Poxvirology
    Subtitle of host publicationMethods and Protocols
    PublisherHumana Press Inc.
    Number of pages20
    ISBN (Print)9781617798757
    Publication statusPublished - 2012

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745


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