Anonymous nuclear markers for the eastern fence lizard, Sceloporus undulatus

E. B. Rosenblum; N. M. Belfiore; C. Moritz

doi:10.1111/j.1471-8286.2006.01547.x

Anonymous nuclear markers for the eastern fence lizard, Sceloporus undulatus

E. B. Rosenblum^*, N. M. Belfiore, C. Moritz

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

We present results from a screen for de novo variable nuclear loci using a genomic library approach in Sceloporus undulatus, the eastern fence lizard. We tested amplification success for 77 primer pairs in S. undulatus, Sceloporus occidentalis and Sceloporus grammicus. Many loci amplified in all three species suggesting that our primers will be useful for developing sequencing or single nucleotide polymorphism (SNP) genotyping markers in other sceloporine lizards. We also sequenced 19 loci, containing 158 variable sites, for 91 S. undulatus individuals. We report high levels of nucleotide variation in this species with an average of 38 SNPs per kilobase.

Original language	English
Pages (from-to)	113-116
Number of pages	4
Journal	Molecular Ecology Notes
Volume	7
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01547.x
Publication status	Published - Jan 2007
Externally published	Yes

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title = "Anonymous nuclear markers for the eastern fence lizard, Sceloporus undulatus",

abstract = "We present results from a screen for de novo variable nuclear loci using a genomic library approach in Sceloporus undulatus, the eastern fence lizard. We tested amplification success for 77 primer pairs in S. undulatus, Sceloporus occidentalis and Sceloporus grammicus. Many loci amplified in all three species suggesting that our primers will be useful for developing sequencing or single nucleotide polymorphism (SNP) genotyping markers in other sceloporine lizards. We also sequenced 19 loci, containing 158 variable sites, for 91 S. undulatus individuals. We report high levels of nucleotide variation in this species with an average of 38 SNPs per kilobase.",

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author = "Rosenblum, {E. B.} and Belfiore, {N. M.} and C. Moritz",

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doi = "10.1111/j.1471-8286.2006.01547.x",

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AB - We present results from a screen for de novo variable nuclear loci using a genomic library approach in Sceloporus undulatus, the eastern fence lizard. We tested amplification success for 77 primer pairs in S. undulatus, Sceloporus occidentalis and Sceloporus grammicus. Many loci amplified in all three species suggesting that our primers will be useful for developing sequencing or single nucleotide polymorphism (SNP) genotyping markers in other sceloporine lizards. We also sequenced 19 loci, containing 158 variable sites, for 91 S. undulatus individuals. We report high levels of nucleotide variation in this species with an average of 38 SNPs per kilobase.

KW - Nucleotide diversity

KW - Sceloporus

KW - Single nucleotide polymorphism

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JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

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ER -

Anonymous nuclear markers for the eastern fence lizard, Sceloporus undulatus

Abstract

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