Anti-inflammatory Activity, Cytotoxicity and Active Compounds of Tinospora smilacina Benth

Rachel W. Li; David N. Leach; Stephen P. Myers; Gregory J. Leach; G. David Lin; Donald J. Brushett; Peter G. Waterman

doi:10.1002/ptr.1373

Anti-inflammatory Activity, Cytotoxicity and Active Compounds of Tinospora smilacina Benth

Rachel W. Li^*, David N. Leach, Stephen P. Myers, Gregory J. Leach, G. David Lin, Donald J. Brushett, Peter G. Waterman

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

Tinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inflammatory disorders. As part of an investigation into the anti-inflammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efficacy and safety of Tinospora smilacina. An ethanol extract of this plant was evaluated in vitro for anti-inflammatory activities on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LO) and phospholipase A₂ (PA₂). The ethanol extract of Tinospora smilacina showed inhibitory activities on COX-1, COX-2, 5-LO and PA₂ with the IC₅₀ values of 63.5, 81.2, 92.1 and 30.5 μg/mL respectively. Cytotoxic effect of the extracts of Tinospora smilacina was investigated in vitro using ATP-based luminescence assay and the results showed no cytotoxic effect on cell lines of skin fibroblasts (1BR3), human Caucasian hepatocyte carcinoma (Hep G2) and human Caucasian promyelocytic leukaemia (HL-60). This paper also describes the results of fractionations and bioassay guided chemical studies, suggesting that the anti- inflammatory activity is due to triterpene-fatty acid esters and free fatty acids.

Original language	English
Pages (from-to)	78-83
Number of pages	6
Journal	Phytotherapy Research
Volume	18
Issue number	1
DOIs	https://doi.org/10.1002/ptr.1373
Publication status	Published - Jan 2004
Externally published	Yes

Access to Document

10.1002/ptr.1373

Cite this

@article{941ee111ebae4b09930174ddc7394752,

title = "Anti-inflammatory Activity, Cytotoxicity and Active Compounds of Tinospora smilacina Benth",

abstract = "Tinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inflammatory disorders. As part of an investigation into the anti-inflammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efficacy and safety of Tinospora smilacina. An ethanol extract of this plant was evaluated in vitro for anti-inflammatory activities on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LO) and phospholipase A2 (PA2). The ethanol extract of Tinospora smilacina showed inhibitory activities on COX-1, COX-2, 5-LO and PA2 with the IC50 values of 63.5, 81.2, 92.1 and 30.5 μg/mL respectively. Cytotoxic effect of the extracts of Tinospora smilacina was investigated in vitro using ATP-based luminescence assay and the results showed no cytotoxic effect on cell lines of skin fibroblasts (1BR3), human Caucasian hepatocyte carcinoma (Hep G2) and human Caucasian promyelocytic leukaemia (HL-60). This paper also describes the results of fractionations and bioassay guided chemical studies, suggesting that the anti- inflammatory activity is due to triterpene-fatty acid esters and free fatty acids.",

keywords = "5-lipoxygenase, Anti-inflammatory activity, Cycloxygenase-1 and -2, Cytotoxicity, LC-MS, NMR, Tinospora smilacina",

author = "Li, {Rachel W.} and Leach, {David N.} and Myers, {Stephen P.} and Leach, {Gregory J.} and Lin, {G. David} and Brushett, {Donald J.} and Waterman, {Peter G.}",

year = "2004",

month = jan,

doi = "10.1002/ptr.1373",

language = "English",

volume = "18",

pages = "78--83",

journal = "Phytotherapy Research",

issn = "0951-418X",

publisher = "John Wiley & Sons Ltd.",

number = "1",

}

TY - JOUR

T1 - Anti-inflammatory Activity, Cytotoxicity and Active Compounds of Tinospora smilacina Benth

AU - Li, Rachel W.

AU - Leach, David N.

AU - Myers, Stephen P.

AU - Leach, Gregory J.

AU - Lin, G. David

AU - Brushett, Donald J.

AU - Waterman, Peter G.

PY - 2004/1

Y1 - 2004/1

N2 - Tinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inflammatory disorders. As part of an investigation into the anti-inflammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efficacy and safety of Tinospora smilacina. An ethanol extract of this plant was evaluated in vitro for anti-inflammatory activities on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LO) and phospholipase A2 (PA2). The ethanol extract of Tinospora smilacina showed inhibitory activities on COX-1, COX-2, 5-LO and PA2 with the IC50 values of 63.5, 81.2, 92.1 and 30.5 μg/mL respectively. Cytotoxic effect of the extracts of Tinospora smilacina was investigated in vitro using ATP-based luminescence assay and the results showed no cytotoxic effect on cell lines of skin fibroblasts (1BR3), human Caucasian hepatocyte carcinoma (Hep G2) and human Caucasian promyelocytic leukaemia (HL-60). This paper also describes the results of fractionations and bioassay guided chemical studies, suggesting that the anti- inflammatory activity is due to triterpene-fatty acid esters and free fatty acids.

AB - Tinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inflammatory disorders. As part of an investigation into the anti-inflammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efficacy and safety of Tinospora smilacina. An ethanol extract of this plant was evaluated in vitro for anti-inflammatory activities on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LO) and phospholipase A2 (PA2). The ethanol extract of Tinospora smilacina showed inhibitory activities on COX-1, COX-2, 5-LO and PA2 with the IC50 values of 63.5, 81.2, 92.1 and 30.5 μg/mL respectively. Cytotoxic effect of the extracts of Tinospora smilacina was investigated in vitro using ATP-based luminescence assay and the results showed no cytotoxic effect on cell lines of skin fibroblasts (1BR3), human Caucasian hepatocyte carcinoma (Hep G2) and human Caucasian promyelocytic leukaemia (HL-60). This paper also describes the results of fractionations and bioassay guided chemical studies, suggesting that the anti- inflammatory activity is due to triterpene-fatty acid esters and free fatty acids.

KW - 5-lipoxygenase

KW - Anti-inflammatory activity

KW - Cycloxygenase-1 and -2

KW - Cytotoxicity

KW - LC-MS

KW - NMR

KW - Tinospora smilacina

UR - http://www.scopus.com/inward/record.url?scp=0842291442&partnerID=8YFLogxK

U2 - 10.1002/ptr.1373

DO - 10.1002/ptr.1373

M3 - Article

SN - 0951-418X

VL - 18

SP - 78

EP - 83

JO - Phytotherapy Research

JF - Phytotherapy Research

IS - 1

ER -

Anti-inflammatory Activity, Cytotoxicity and Active Compounds of Tinospora smilacina Benth

Abstract

Access to Document

Other files and links

Fingerprint

Cite this