Anticipating and Identifying Collateral Damage in Genome Editing

Gaëtan Burgio; Lydia Teboul

doi:10.1016/j.tig.2020.09.011

Anticipating and Identifying Collateral Damage in Genome Editing

Gaëtan Burgio^*, Lydia Teboul

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

31 Citations (Scopus)

Abstract

Genome editing has powerful applications in research, healthcare, and agriculture. However, the range of possible molecular events resulting from genome editing has been underestimated and the technology remains unpredictable on, and away from, the target locus. This has considerable impact in providing a safe approach for therapeutic genome editing, agriculture, and other applications. This opinion article discusses how to anticipate and detect those editing events by a combination of assays to capture all possible genomic changes. It also discusses strategies for preventing unwanted effects, critical to appraise the benefit or risk associated with the use of the technology. Anticipating and verifying the result of genome editing are essential for the success for all applications.

Original language	English
Pages (from-to)	905-914
Number of pages	10
Journal	Trends in Genetics
Volume	36
Issue number	12
DOIs	https://doi.org/10.1016/j.tig.2020.09.011
Publication status	Published - Dec 2020

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title = "Anticipating and Identifying Collateral Damage in Genome Editing",

abstract = "Genome editing has powerful applications in research, healthcare, and agriculture. However, the range of possible molecular events resulting from genome editing has been underestimated and the technology remains unpredictable on, and away from, the target locus. This has considerable impact in providing a safe approach for therapeutic genome editing, agriculture, and other applications. This opinion article discusses how to anticipate and detect those editing events by a combination of assays to capture all possible genomic changes. It also discusses strategies for preventing unwanted effects, critical to appraise the benefit or risk associated with the use of the technology. Anticipating and verifying the result of genome editing are essential for the success for all applications.",

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year = "2020",

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AU - Burgio, Gaëtan

AU - Teboul, Lydia

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N2 - Genome editing has powerful applications in research, healthcare, and agriculture. However, the range of possible molecular events resulting from genome editing has been underestimated and the technology remains unpredictable on, and away from, the target locus. This has considerable impact in providing a safe approach for therapeutic genome editing, agriculture, and other applications. This opinion article discusses how to anticipate and detect those editing events by a combination of assays to capture all possible genomic changes. It also discusses strategies for preventing unwanted effects, critical to appraise the benefit or risk associated with the use of the technology. Anticipating and verifying the result of genome editing are essential for the success for all applications.

AB - Genome editing has powerful applications in research, healthcare, and agriculture. However, the range of possible molecular events resulting from genome editing has been underestimated and the technology remains unpredictable on, and away from, the target locus. This has considerable impact in providing a safe approach for therapeutic genome editing, agriculture, and other applications. This opinion article discusses how to anticipate and detect those editing events by a combination of assays to capture all possible genomic changes. It also discusses strategies for preventing unwanted effects, critical to appraise the benefit or risk associated with the use of the technology. Anticipating and verifying the result of genome editing are essential for the success for all applications.

KW - ectopic insertions

KW - genome editing

KW - off-target activity

KW - on-target activity

KW - validation

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DO - 10.1016/j.tig.2020.09.011

M3 - Review article

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SP - 905

EP - 914

JO - Trends in Genetics

JF - Trends in Genetics

IS - 12

ER -

Anticipating and Identifying Collateral Damage in Genome Editing

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