TY - JOUR
T1 - Antileishmanial polyphenols from Corymbia maculata
AU - Sidana, Jasmeen
AU - Neeradi, Dinesh
AU - Choudhary, Alka
AU - Singh, Sushma
AU - Foley, William J.
AU - Singh, Inder Pal
PY - 2013/7
Y1 - 2013/7
N2 - An activity-guided fractionation was used to identify the antileishmanial compounds of Corymbia maculata. The hexane, ethyl acetate and methanol extracts were active in in vitro antileishmanial assay. Twelve polyphenols including 8-demethyl eucalyptin (1), eucalyptin (2), myrciaphenone A (3), myrciaphenone B (4), quercetin-3-O-β-d-xylopyranoside (5), myricetin-3-O-α-l- rhamnopyranoside (6), quercetin-3-O-β-d-galactopyranoside (7), quercetin-3-O-β-d-glucopyranoside (8), quercetin-3-O-α-l- rhamnopyranoside (9), syringic acid (10), gallic acid-3-methyl ether (11), gallic acid-4-methyl ether (12) and gallic acid (13) were isolated from the active extracts. All the tested compounds except 8-demethyleucalyptin and myrciaphenone B showed strong to moderate (6.9-24.5 μM) antileishmanial activity against Leishmania donovani promastigotes. An HPLC-PDA method has been developed to detect/quantify 29 compounds in the extracts of C. maculata leaves. This validated method allows simultaneous quantitation of seven flavonoids, fourteen phloroglucinols and eight other polyphenols and can be applied for qualitative as well as quantitative determination of phytoconstituents in Eucalyptus matrices. [Figure not available: see fulltext.]
AB - An activity-guided fractionation was used to identify the antileishmanial compounds of Corymbia maculata. The hexane, ethyl acetate and methanol extracts were active in in vitro antileishmanial assay. Twelve polyphenols including 8-demethyl eucalyptin (1), eucalyptin (2), myrciaphenone A (3), myrciaphenone B (4), quercetin-3-O-β-d-xylopyranoside (5), myricetin-3-O-α-l- rhamnopyranoside (6), quercetin-3-O-β-d-galactopyranoside (7), quercetin-3-O-β-d-glucopyranoside (8), quercetin-3-O-α-l- rhamnopyranoside (9), syringic acid (10), gallic acid-3-methyl ether (11), gallic acid-4-methyl ether (12) and gallic acid (13) were isolated from the active extracts. All the tested compounds except 8-demethyleucalyptin and myrciaphenone B showed strong to moderate (6.9-24.5 μM) antileishmanial activity against Leishmania donovani promastigotes. An HPLC-PDA method has been developed to detect/quantify 29 compounds in the extracts of C. maculata leaves. This validated method allows simultaneous quantitation of seven flavonoids, fourteen phloroglucinols and eight other polyphenols and can be applied for qualitative as well as quantitative determination of phytoconstituents in Eucalyptus matrices. [Figure not available: see fulltext.]
KW - Corymbia maculata
KW - HPLC quantitation
KW - flavonoid glycosides
KW - methylated flavonoids
KW - myrciaphenone
UR - http://www.scopus.com/inward/record.url?scp=84883638523&partnerID=8YFLogxK
U2 - 10.1007/s12039-013-0440-8
DO - 10.1007/s12039-013-0440-8
M3 - Article
SN - 0974-3626
VL - 125
SP - 765
EP - 775
JO - Journal of Chemical Sciences
JF - Journal of Chemical Sciences
IS - 4
ER -