Arbitrary multisite two-photon excitation in four dimensions

Vincent Ricardo Daria; Christian Stricker; Richard Bowman; Stephen Redman; Hans A. Bachor

doi:10.1063/1.3216581

Arbitrary multisite two-photon excitation in four dimensions

Vincent Ricardo Daria, Christian Stricker, Richard Bowman, Stephen Redman, Hans A. Bachor

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.

Original language	English
Article number	093701
Journal	Applied Physics Letters
Volume	95
Issue number	9
DOIs	https://doi.org/10.1063/1.3216581
Publication status	Published - 2009

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AB - We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.

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Arbitrary multisite two-photon excitation in four dimensions

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