Assembling branched and macrocyclic peptides on proteins

Sven Ullrich; Santhanalaxmi Kumaresan; Marina G. Rahman; Bishvanwesha Panda; Richard Morewood; Christoph Nitsche

doi:10.1039/d4cc06442a

Assembling branched and macrocyclic peptides on proteins

Sven Ullrich, Santhanalaxmi Kumaresan, Marina G. Rahman, Bishvanwesha Panda, Richard Morewood, Christoph Nitsche

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Abstract

A two-step, biocompatible strategy enables site-specific generation of branched and macrocyclic peptide-protein conjugates. Solvent-exposed cysteines on proteins are modified by a small bifunctional reagent at near-physiological pH, followed by cyanopyridine-aminothiol click reactions to create branched or macrocyclic peptide architectures. This method offers design strategies for next-generation protein therapeutics.

Original language	English
Number of pages	4
Journal	Chemical Communications
Issue number	14
Early online date	Jan 2025
DOIs	https://doi.org/10.1039/d4cc06442a
Publication status	Published - 17 Jan 2025

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10.1039/d4cc06442a

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AU - Nitsche, Christoph

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Assembling branched and macrocyclic peptides on proteins

Abstract

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