TY - JOUR
T1 - Association of NLRP3 single nucleotide polymorphisms with ulcerative colitis
T2 - A case-control study
AU - Hanaei, S.
AU - Sadr, M.
AU - Rezaei, A.
AU - Shahkarami, S.
AU - Ebrahimi Daryani, N.
AU - Bidoki, A. Z.
AU - Rezaei, N.
N1 - Publisher Copyright:
© 2017 Elsevier Masson SAS
PY - 2018/6
Y1 - 2018/6
N2 - Background: Ulcerative colitis (UC) is inflammatory bowel disease (IBD), characterized by chronic inflammation episodes within mucosal layer of the intestine mostly affecting colon and rectum. As the role of innate immunity in pathogenesis of disease and important role of NLRP3, the aim of this study is to investigate the association of NLRP3 SNPs with UC in Iranian patients. Methods: Blood samples from 45 UC patients and 56 healthy subjects were tested for single nucleotide polymorphisms in rs10754558, rs3806265, rs4612666, and rs35829419 of NLRP3 gene, using real-time PCR method. Results: Among the investigated SNPs, “GG” genotype of rs10754558 have been 2.48 times more common among UC patients (P = 0.04), while “CG” genotype has indicated protective effect against UC, as more frequently found in healthy subjects. Conclusion: Despite no significant association between three investigated SNPs and disease, “GG” and “CG” genotypes of rs10754558 have been significantly associated with disease.
AB - Background: Ulcerative colitis (UC) is inflammatory bowel disease (IBD), characterized by chronic inflammation episodes within mucosal layer of the intestine mostly affecting colon and rectum. As the role of innate immunity in pathogenesis of disease and important role of NLRP3, the aim of this study is to investigate the association of NLRP3 SNPs with UC in Iranian patients. Methods: Blood samples from 45 UC patients and 56 healthy subjects were tested for single nucleotide polymorphisms in rs10754558, rs3806265, rs4612666, and rs35829419 of NLRP3 gene, using real-time PCR method. Results: Among the investigated SNPs, “GG” genotype of rs10754558 have been 2.48 times more common among UC patients (P = 0.04), while “CG” genotype has indicated protective effect against UC, as more frequently found in healthy subjects. Conclusion: Despite no significant association between three investigated SNPs and disease, “GG” and “CG” genotypes of rs10754558 have been significantly associated with disease.
KW - NLRP3
KW - Single nucleotide polymorphisms
KW - Ulcerative colitis
UR - http://www.scopus.com/inward/record.url?scp=85034964699&partnerID=8YFLogxK
U2 - 10.1016/j.clinre.2017.09.003
DO - 10.1016/j.clinre.2017.09.003
M3 - Article
SN - 2210-7401
VL - 42
SP - 269
EP - 275
JO - Clinics and Research in Hepatology and Gastroenterology
JF - Clinics and Research in Hepatology and Gastroenterology
IS - 3
ER -