Axonal transport of neuronal calcium sensor-1 and phosphatidylinositol 4-kinaseβ in the adult rat sciatic nerve

Neuronal calcium sensor-1 (NCS-1) and its putative substrate phosphatidylinositol 4-kinaseβ (Ptdlns 4-kinaseβ) both indirectly regulate synaptic vesicle exocytosis and are located in DRG neurites. In this study we have tested whether NCS-1 and Ptdlns 4-kinaseβ are transported in axons using the analysis of double ligation approach in the adult rat sciatic nerve. We show that NCS-1 accumulates on both the distal and proximal side of the nerve ligation indicating that this protein undergoes bidirectional transport in axons. In contrast, Ptdlns 4-kinaseβ accumulated on the distal side which suggests that it undergoes retrograde axonal transport and unlike NCS-1 was also present in non-neuronal cells. (C) 2000 Lippincott Williams and Wilkins.