Backbone assignment of fully protonated solid proteins by 1H detection and ultrafast magic-angle-spinning NMR spectroscopy

Alessandro Marchetti, Stefan Jehle, Michele Felletti, Michael J. Knight, Yao Wang, Zhi Qiang Xu, Ah Young Park, Gottfried Otting, Anne Lesage, Lyndon Emsley, Nicholas E. Dixon, Guido Pintacuda*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    86 Citations (Scopus)

    Abstract

    Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins can be analyzed without any need for deuteration of the protein sample. This approach provides assignments of the backbone 1H, 15N, 13C α, and 13CO resonances and yields information about 1H-1H proximities.

    Original languageEnglish
    Pages (from-to)10756-10759
    Number of pages4
    JournalAngewandte Chemie - International Edition
    Volume51
    Issue number43
    DOIs
    Publication statusPublished - 22 Oct 2012

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