Bicarbonate exchange kinetics at equilibrium across the erythrocyte membrane by 13C NMR

Bogdan E. Chapman; Kiaran Kirk; Philip W. Kuchel

doi:10.1016/0006-291X(86)90904-6

Bicarbonate exchange kinetics at equilibrium across the erythrocyte membrane by ¹³C NMR

Bogdan E. Chapman^*, Kiaran Kirk, Philip W. Kuchel

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The rate of exchange of ¹³C-labelled bicarbonate across the membranes of human erythrocytes in suspension, at thermal and chemical equilibrium, was measured using ¹³C NMR spectroscopy: the permeability coefficient (3.34 × 10^-4 cm s^-1) agrees well with previous values obtained with other methods. Data analysis was complicated by the need to consider the Donnan ratio of the charged species inside and outside the cells. This work appears to be the first, involving the present NMR procedure, for studying fast membrane transport of a molecule other than water.

Original language	English
Pages (from-to)	266-272
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	136
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(86)90904-6
Publication status	Published - 14 Apr 1986
Externally published	Yes

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title = "Bicarbonate exchange kinetics at equilibrium across the erythrocyte membrane by 13C NMR",

abstract = "The rate of exchange of 13C-labelled bicarbonate across the membranes of human erythrocytes in suspension, at thermal and chemical equilibrium, was measured using 13C NMR spectroscopy: the permeability coefficient (3.34 × 10-4 cm s-1) agrees well with previous values obtained with other methods. Data analysis was complicated by the need to consider the Donnan ratio of the charged species inside and outside the cells. This work appears to be the first, involving the present NMR procedure, for studying fast membrane transport of a molecule other than water.",

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AU - Chapman, Bogdan E.

AU - Kirk, Kiaran

AU - Kuchel, Philip W.

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Y1 - 1986/4/14

N2 - The rate of exchange of 13C-labelled bicarbonate across the membranes of human erythrocytes in suspension, at thermal and chemical equilibrium, was measured using 13C NMR spectroscopy: the permeability coefficient (3.34 × 10-4 cm s-1) agrees well with previous values obtained with other methods. Data analysis was complicated by the need to consider the Donnan ratio of the charged species inside and outside the cells. This work appears to be the first, involving the present NMR procedure, for studying fast membrane transport of a molecule other than water.

AB - The rate of exchange of 13C-labelled bicarbonate across the membranes of human erythrocytes in suspension, at thermal and chemical equilibrium, was measured using 13C NMR spectroscopy: the permeability coefficient (3.34 × 10-4 cm s-1) agrees well with previous values obtained with other methods. Data analysis was complicated by the need to consider the Donnan ratio of the charged species inside and outside the cells. This work appears to be the first, involving the present NMR procedure, for studying fast membrane transport of a molecule other than water.

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U2 - 10.1016/0006-291X(86)90904-6

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JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Bicarbonate exchange kinetics at equilibrium across the erythrocyte membrane by ¹³C NMR

Abstract

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