TY - JOUR
T1 - Bulk segregation mapping of mutations in closely related strains of mice
AU - Xia, Yu
AU - Won, Sungyong
AU - Du, Xin
AU - Lin, Pei
AU - Ross, Charles
AU - La Vine, Diantha
AU - Wiltshire, Sean
AU - Leiva, Gabriel
AU - Vidal, Silvia M.
AU - Whittle, Belinda
AU - Goodnow, Christopher C.
AU - Koziol, James
AU - Moresco, Eva Marie Y.
AU - Beutler, Bruce
PY - 2010/12
Y1 - 2010/12
N2 - Phenovariance may be obscured when genetic mapping is performed using highly divergent strains, and closely similar strains are preferred if adequate marker density can be established. We sequenced the C57BL/10J mouse genome using the Applied Biosystems SOLiD platform and here describe a genomewide panel of informative markers that permits the mapping of mutations induced on the closely related C57BL/6J background by outcrossing to C57BL/10J, and backcrossing or intercrossing. The panel consists of 127 single nucleotide polymorphisms validated by capillary sequencing: 124 spaced at ∼20-Mb intervals across the 19 autosomes, and three markers on the X chromosome. We determined the genetic relationship between four C57BL-derived substrains and used the panel to map two N-ethyl-N-nitrosourea (ENU)-induced mutations responsible for visible phenotypes in C57BL/6J mice through bulk segregation analysis. Capillary sequencing, with computation of relative chromatogram peak heights, was used to determine the proportion of alleles from each strain at each marker.
AB - Phenovariance may be obscured when genetic mapping is performed using highly divergent strains, and closely similar strains are preferred if adequate marker density can be established. We sequenced the C57BL/10J mouse genome using the Applied Biosystems SOLiD platform and here describe a genomewide panel of informative markers that permits the mapping of mutations induced on the closely related C57BL/6J background by outcrossing to C57BL/10J, and backcrossing or intercrossing. The panel consists of 127 single nucleotide polymorphisms validated by capillary sequencing: 124 spaced at ∼20-Mb intervals across the 19 autosomes, and three markers on the X chromosome. We determined the genetic relationship between four C57BL-derived substrains and used the panel to map two N-ethyl-N-nitrosourea (ENU)-induced mutations responsible for visible phenotypes in C57BL/6J mice through bulk segregation analysis. Capillary sequencing, with computation of relative chromatogram peak heights, was used to determine the proportion of alleles from each strain at each marker.
UR - http://www.scopus.com/inward/record.url?scp=78650540549&partnerID=8YFLogxK
U2 - 10.1534/genetics.110.121160
DO - 10.1534/genetics.110.121160
M3 - Article
SN - 0016-6731
VL - 186
SP - 1139
EP - 1145
JO - Genetics
JF - Genetics
IS - 4
ER -